Differential expression of extracellular matrix metalloproteinase inducer (EMMPRIN/CD147) in avian tibial dyschondroplasia.

Differential expression of extracellular matrix metalloproteinase inducer (EMMPRIN/CD147) in avian tibial dyschondroplasia. Avian Pathol. 2014 Nov 17;:1 Authors: Shahzad M, Liu J, Gao J, Wang Z, Zhang D, Nabi F, Li K, Li J Abstract Tibial dyschondroplasia (TD) is an avian bone disorder of different aetiologies that may be associated with lameness. It is characterized by focal disruption of endochondral bone formation, with a lack of matrix proteolysis and an accumulation of non-mineralized avascular cartilage. The aim of this study was to determine the expression of extracellular matrix metalloproteinase inducer (EMMPRIN/CD147) in normal, thiram-induced TD lesions and in the process of recovery from TD in broiler chickens. An extracellular matrix (ECM) degrading enzyme [matrix metalloproteinase-9 (MMP-9)] was selected to investigate the effects of CD147 in the degradation of ECM. Gene expression was analysed by quantitative real-time PCR and protein levels by immunohistochemistry and western blotting. The birds were divided into three groups: (1) thiram fed; (2) recovery; and (3) controls. Genes encoding CD147 and MMP-9 were down-regulated during the development of the disease, and up-regulated during recovery. Western blotting also showed lower protein levels of CD147 in TD which increased during the recovery phase associated with ECM degradation and growth plate repair. The findings of this study suggest that ECM has crucial role i...
Source: Avian Pathology - Category: Pathology Authors: Tags: Avian Pathol Source Type: research