Combined effects of dietary bovine lactoferrin, Lactobacillus plantarum , and xylooligosaccharide on hemato-immunological and digestive enzymes of silvery-black porgy ( Sparidentex hasta ) fingerlings

AbstractA 6-week study was conducted to evaluate the effects of the following eight experimental diets, which varied in bovine lactoferrin (LF), xylooligosacharide (XOS), andLactobacillus plantarum (LP) as probiotic silvery-black porgy (Sparidentex hasta) juveniles including control (diet 1), 400  mg kg−1 LF + 5  g kg−1 XOS (diet 2), 400  mg kg−1 LF + 10  g kg−1 XOS (diet 3), 400  mg kg−1 LF + 1  × 106 CFU g−1 LP (diet 4), 800  mg kg−1 LF + 5  g kg−1 XOS (diet 5), 800  mg kg−1 LF + 10  g kg−1 XOS (diet 6), and 400  mg kg−1 LF + 1  × 106 CFU g−1 LP (diet 7). Growth performance and humoral immune responses were not affected by different experimental groups (P >  0.05). Hematological parameters including red blood cell count, hemoglobin content, and hematocrit increased by supplementing diets with different combinations of immunostimulants (P <  0.05). Fish fed diet supplemented with 800 mg kg−1 LF + 5  g kg−1 XOS had highest total protease (1.0  ± 0.1 U mg protein−1) and α-amylase (34.9 ± 2.3 mg protein−1) activities (P <  0.05). Our results suggest that diets supplemented with selected levels of LF, XOS, andL. plantarum could not improve growth performance and humoral immunity, however improved hematological and digestive enzyme activities inS. hasta fingerlings.
Source: Comparative Clinical Pathology - Category: Pathology Source Type: research