Subclinical infections of Anaplasma phagocytophilum and Anaplasma bovis in dogs in Ibaraki, Japan.
Subclinical infections of Anaplasma phagocytophilum and Anaplasma bovis in dogs in Ibaraki, Japan.
Jpn J Infect Dis. 2019 Jan 31;:
Authors: Fukui Y, Inokuma H
Abstract
The prevalence of Anaplasma infection in 332 dogs from Ibaraki, Japan, was evaluated by serological and molecular surveys. Immunofluorescence antibody assay (IFA) against Anaplasma phagocytophilum indicated that 7 (2.1%) of 328 dogs were positive for A. phagocytophilum. Screening PCR demonstrated that 8 (2.4%) of 331 dogs were positive for Anaplasmataceae. Phylogenetic analysis of the partial 16S rRNA sequence of PCR amplicons revealed that 6 sequences were most similar to the 16S rRNA sequence of Wolbachia sp. and the remaining 2 to Anaplasma bovis. Further analysis by A. phagocytophilum-specific nested PCR demonstrated that 1 dog infected with A. bovis was also positive for A. phagocytophilum. This is the first study to report the dual infection of a dog in Japan with A. bovis and A. phagocytophilum.
PMID: 30700657 [PubMed - as supplied by publisher]
Source: Japanese Journal of Infectious Diseases - Category: Infectious Diseases Authors: Fukui Y, Inokuma H Tags: Jpn J Infect Dis Source Type: research