Effect of Crotalaria lachnosema Stapf . (Fabaceae) extract on biomarkers of hepatic and renal function and oxidative stress in male Wistar rats

AbstractThe aim of this study was to determine the subchronic effect ofCrotalaria lachnosema Stapf. (Fabaceae) extract (MLECL) on biochemical and oxidative stress biomarkers in male Wistar rats. The rats were assigned into four groups designated as groups 1(control), 2, 3, and 4, of ten rats each, and were exposed to dose levels of 0, 40, 200, and 1000  mg of MLECL extract per kilogram in feed, respectively, for a period of 6 weeks. At the end of the study period, the surviving rats were sacrificed and blood was collected from each rat via jugular venesection and used for biochemical analysis. Postmortem examination was carried out on euthanized rats after which tissue sections of various organs were harvested for histopathological examination. Exposure to the extract was found to significantly (p <  0.0001) increased the values of urea and creatinine in groups 2, 3, and 4 (urea, 4.32 ± 0.15, 3.92 ± 0.32, 4.14 ± 0.09 mmol/l and creatinine, 68.60 ± 1.99, 89.40 ± 5.61, 71.60 ± 3.67 μmol/l, respectively) when compared with group 1 (urea, 2.62 ± 0.10 mmol/l, creatinine, 42.80 ± 1.59 μmol/l, respectively). Similarly, the extract was found to significantly affect the serum levels of liver enzymes only in group 4 (ALT, 52.80 ± 2.11 IU/L; AST, 49.40 ± 2.25 IU/L; ALP, 92.20 ± 8.24 IU/L), (p <  0.05) than the corresponding values in the control group (ALT, 45.00 ± 1.52 IU/L; AST, 38.00 ± 1.52 IU...
Source: Comparative Clinical Pathology - Category: Pathology Source Type: research