In vitro and In vivo toxicity assessment of phytofabricated ZnO nanoparticles showing bacteriostatic effect and larvicidal efficacy against Culex quinquefasciatus

Publication date: Available online 30 January 2019Source: Journal of Photochemistry and Photobiology B: BiologyAuthor(s): Mariappan Yazhiniprabha, Baskaralingam Vaseeharan, Avinash Sonawane, Ananyaashree BeheraAbstractMurraya koenigii berry extract based zinc oxide nanoparticles (Mk-ZnO NPs) were synthesized by simple co-precipitation method and examined for bacteriostatic and larvicidal efficiency. Synthesized Mk-ZnO NPs were characterized by UV–Vis spectroscopy at 336 nm. X-Ray diffraction (XRD) showed crystalline nature as hexagonal. Fourier transform infrared spectroscopy (FTIR) spectrum exhibited strong peak at 3442.80 cm−1. Field emission scanning electron microscopy (FE-SEM) showed hexagonal shape of the particle. Transmission electron microscopy (TEM) measured 10–15 nm sized Mk-ZnO NPs. EDX peaks confirm 71.99% of zinc and 11.42% of oxide in Mk-ZnO NPs. Minimum inhibitory concentration (MIC) analysis reveals Mk-ZnO NPs inhibit growth of Gram positive (Staphylococcus aureus, Lysinibacillus fusiformis) and Gram negative (Proteus vulgaris, Providencia vermicola) bacteria at 40 and 50 μg mL−1 respectively. Live & dead assay confirms that Mk-ZnO NPs inhibits bacterial growth at 50 μg mL−1. Bacterial biofilm thickness significantly reduced by Mk-ZnO NPs at 50 μg mL−1. In vitro toxicity of Mk-ZnO NPs on RAW 264.7 macrophages determines 90–50% cell viability at concentrations of 10–100 μg mL−1. In vivo toxicity assay results indicat...
Source: Journal of Photochemistry and Photobiology B: Biology - Category: Speech-Language Pathology Source Type: research