Determination of volatile organic compounds in SW620 colorectal cancer cells and tumor-bearing mice

Publication date: Available online 30 January 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Guiyue Wang, Yuhang Li, Miao Liu, Nana Guo, Ci Han, Desheng Liu, Dandan Li, Mengyuan Yang, Yahui Peng, Yansong Liu, Kaijiang Yu, Changsong WangAbstractEarly diagnosis and early treatment are important factors in reducing colorectal cancer (CRC) metastasis and mortality. Volatile organic compounds (VOCs) released by the human body have great potential for use in clinical diagnosis and therapeutic monitoring for CRC. The aim of our study was to identify VOCs with high specificity and high sensitivity for CRC and to provide a method for early diagnosis of CRC. Gas chromatography-mass spectrometry (GC-MS) was utilized to analyze metabolites in both the in vivo and in vitro experimental groups. In vivo, VOCs were analyzed in the blood of mice after cell inoculation and tumor resection. In vitro experiments were performed by comparing changes in VOCs in an HCoEpiC cell group, control group, SW620 cell group and Arsenic trioxide + SW620 group. We observed changes in VOCs in a series of CRC SW620 cells in vivo and in vitro. Among these changes, we found that the concentrations of 8 substances, including acetone, increased with tumor growth. Nine substances were found to be significantly elevated in the SW620 cancer cell group compared with the other groups. Only one substance was consumed by the tumor in both the in vivo and in vitro experiments. Our stu...
Source: Journal of Pharmaceutical and Biomedical Analysis - Category: Drugs & Pharmacology Source Type: research

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Publication date: Available online 18 November 2019Source: Colloids and Surfaces A: Physicochemical and Engineering AspectsAuthor(s): Juan Liao, Siyu Peng, Mei Long, Yaya Zhang, Huaming Yang, Yi Zhang, Jing HuangAbstractNatural halloysite clay nanotubes (halloysite nanotubes, HNTs) are potentially used in colon cancer therapy owing to their excellent physicochemical properties. However, the size-dependent interaction between HNTs and colon cancer cells is still an unresolved challenge. Herein, we analyzed the interfacial interactions between HNTs and colon cancer cells, and evaluated the size-dependent biological effects o...
Source: Colloids and Surfaces A: Physicochemical and Engineering Aspects - Category: Chemistry Source Type: research
ConclusionAnalysis of the quality and scientific content of patient information on MIS for CRC revealed a considerable deficiency in the information provided and sub-standard readability. Several aspects such as pre-op preparation, surgical complications and post-operative quality of life which are of concern to the patient were not discussed in a considerable proportion of articles. As the internet is expanding as a readily available source of information, steps should be taken to ensure the highest quality information.
Source: Health Policy and Technology - Category: Health Management Source Type: research
MALAT1 inhibits the Wnt/β-catenin signaling pathway in colon cancer cells and affects cell proliferation and apoptosis. Bosn J Basic Med Sci. 2019 Nov 15;: Authors: Zhang J, Li Q, Xue B, He R Abstract Metastasis associated lung adenocarcinoma transcript 1 (MALAT1) is a highly conserved long noncoding RNA, which has been related to various pathological processes, including cancer. The role and mechanism of MALAT1 in colon cancer are not clear. We investigated MALAT1 expression in colon cancer tissues, the effect of MALAT1 on proliferation and apoptosis of SW480 cells, and the signaling pathway invo...
Source: Bosnian Journal of Basic Medical Sciences - Category: General Medicine Tags: Bosn J Basic Med Sci Source Type: research
Condition:   Colorectal Cancer Metastatic Intervention:   Drug: Oxaliplatin Sponsors:   Criterium, Inc.;   Amgen Not yet recruiting
Source: ClinicalTrials.gov - Category: Research Source Type: clinical trials
Publication date: Available online 18 November 2019Source: Pathology - Research and PracticeAuthor(s): Shiyao Liao, Chang Liu, Guiying Zhu, Kai Wang, Ying Yang, Changmiao WangAbstractE-cadherin and SDC1 are markers of epithelial-to-mesenchymal transition (EMT) that can be used to assess tumour prognosis. SDC1 has different effects in various types of cancers. On the one hand, reduced expression of SDC1 can leads to advantage stages of some cancers, such as gastric and colorectal cancer. On the other hand, SDC1 overexpression can also promote the growth and proliferation of cancer cells in pancreatic and breast cancer. Howe...
Source: Pathology Research and Practice - Category: Pathology Source Type: research
Abstract At present, the strongest evidence for the use of PET/computed tomography (CT) in gastrointestinal (GI) malignancies is to rule out distant metastatic disease at diagnosis, radiation treatment planning for anal malignancies, and disease recurrence monitoring in colorectal and anal malignancies. Use of PET/CT for GI malignancies continues to evolve over time, with new studies evaluating prognostic abilities of PET/CT and with increasing sensitivity and spatial resolution of more modern PET/CT scanners. The authors encourage future applications and prospective evaluation of the use of PET/CT in the staging,...
Source: Clinical Colorectal Cancer - Category: Cancer & Oncology Authors: Tags: PET Clin Source Type: research
Authors: Liu X, Yang G, Huang J, Chai L, Liu X, Dai Q, Yang Z Abstract Aim: KRAS SNPs may increase KRAS transcription and KRAS levels. SNPs of KRAS 3'UTR can affect carcinoma risk and prognosis. Materials &methods: The rs8720 and rs7960917 in KRAS 3'UTR for colorectal carcinoma (CRC) risk and survival were investigated in a case-control study. Association between SNPs and CRC risk, survival analysis were analyzed by an unconditional logistic regression model, log-rank test, Kaplan-Meier estimation, Cox regression model and one-way analysis of variance. Results &conclusion: The genotype CT of rs8720 was sign...
Source: Future Medicine: Biomarkers in Medicine - Category: Internal Medicine Tags: Biomark Med Source Type: research
Conclusion: These results suggest that miR-9-5p targeting of PAK4 could have therapeutic potential for CRC treatment. PMID: 31728150 [PubMed - in process]
Source: Cellular and Molecular Biology Letters - Category: Biochemistry Authors: Tags: Cell Mol Biol Lett Source Type: research
ConclusionsPractical difficulties in quantifying quality of life losses and healthcare expenditures owing to competing risks in life years gained can be overcome. Their inclusion can have a substantial impact on the cost-effectiveness of cancer screening programs.
Source: American Journal of Preventive Medicine - Category: International Medicine & Public Health Source Type: research
Abstract Cloning the open reading frame (ORF) of a protein-coding chimeric RNA into a mammalian expression vector is a simple, practiced way to study the function of the chimeric RNA. Here, we provide procedures for the insertion of the RRM2-C2orf48 fusion ORF into a mammalian expression vector, achieving the overexpression of the RRM2-C2orf48 fusion protein in a colon cancer cell line by retroviral transduction. PMID: 31728969 [PubMed - in process]
Source: Mol Biol Cell - Category: Molecular Biology Authors: Tags: Methods Mol Biol Source Type: research
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