Limitations of diagnostic tests for bacterial infections.

Limitations of diagnostic tests for bacterial infections. Med Mal Infect. 2019 Jan 24;: Authors: Eldin C, Parola P, Raoult D Abstract Lyme disease diagnosis is currently based on serology - an indirect diagnostic method - as laboratory cultures are fastidious. The only direct diagnostic method that can be useful with some specimens (cutaneous biopsies or aspiration fluid) is PCR. We aimed to detail the main limitations of serology and PCR testing in the diagnosis of bacterial infections. Limitations are supported by examples from the recent history of microbiology. The main limitation of bacterial serology is the presence of numerous cross-reactions due to many genes that are common to various bacterial species. Some serological techniques, such as those used for the diagnosis of rickettsioses mainly, have even been based on the existence of cross-reactions. The main limitation of PCR testing is the potential presence of laboratory contaminations. PCR-performing laboratories must therefore be certified for the use of this technique. PCR testing also does not inform on the viability of the identified bacterium and should therefore be interpreted in light of the clinical presentation. These limitations highlight that all diagnostic test results should not be interpreted on their own; the clinical and epidemiological contexts should always be taken into consideration. PMID: 30686500 [PubMed - as supplied by publisher]
Source: Medecine et Maladies Infectieuses - Category: Infectious Diseases Tags: Med Mal Infect Source Type: research

Related Links:

Research ArticlesL. KRBKOV Á, L. HOMOLA, A. HLAVÁČOVÁ, P. MIKOLÁŠEK, J. BEDNÁŘOVÁ, Z. ČERMÁKOVÁ,Epidemiology&Infection,Volume 144 Issue 12, pp 2597-2604Abstract
Source: Epidemiology and Infection - Category: Epidemiology Source Type: research
Kate Winslet picture about renowned fossil hunter had been criticised by relatives for ‘unconfirmed’ portrait of her sexualityThe director of a new biopic about the celebrated fossil hunter Mary Anning has defended his film after criticism of its lesbian romance storyline.Ammonite, which stars Kate Winslet and Saoirse Ronan, recently began filming in Lyme Regis, the coastal town in Dorset where Anning lived in the early 19th century. Anning never married and is not known to have had any direct descendants, but a report in the Telegraph suggested her distant relatives were at odds over the film ’s central ...
Source: Guardian Unlimited Science - Category: Science Authors: Tags: Film Kate Winslet Saoirse Ronan Fossils Biology Culture Evolution Science Sexuality Society Source Type: news
CHICAGO—Keith Winthrop, MD, MPH, professor of public health and preventive medicine at the Oregon Health &Science University School of Medicine, Portland, Ore., began the vaccination session at the 2018 ACR/ARHP Annual Meeting with a review of vaccination timing and targets. He presented a clinically relevant snapshot of the vaccines rheumatologists should consider and emphasized... [Read More]
Source: The Rheumatologist - Category: Rheumatology Authors: Tags: Conditions Meeting Reports 2018 ACR/ARHP Annual Meeting leprosy Lyme Disease vaccination Source Type: research
(Natural News) Most people use essential oils as a mood booster or stress reliever. But according to a study, a potent mix of essential oils from various herbs and medicinal plants can help eliminate the bacteria that cause Lyme disease. The study, which was published in the journal Antibiotics, was conducted by researchers from the Johns Hopkins...
Source: NaturalNews.com - Category: Consumer Health News Source Type: news
Conclusion: This study was the first to determine the frequency of hantavirus in the study region and it includes current data for B. burgdorferi. Consequently, it is recommended that similar studies be carried out on rodents in all the regions at risk. PMID: 30862151 [PubMed - as supplied by publisher]
Source: Turkish Journal of Medical Sciences - Category: General Medicine Tags: Turk J Med Sci Source Type: research
We report nine cases of pediatric neuroborreliosis collected by the National Observatory of Pediatric Bacterial Meningitis in France between 2001 and 2012. The nine children, aged 4-13 years, were identified in northern and eastern France and had the following clinical features: meningeal irritation alone or with facial palsy, or isolated facial palsy. All cases showed anti-Borrelia antibodies in cerebrospinal fluid or serum, or with a positive Borrelia PCR in the CSF. The outcome was favorable in all cases after a 2- to 3-week course of third-generation cephalosporin. On the basis of these nine pediatric cases, this study...
Source: Archives de Pediatrie - Category: Pediatrics Authors: Tags: Arch Pediatr Source Type: research
As I struggle with chronic pain, cozy for me is less hygge and more my ex-boyfriend ’ s mother, nurses with juice and weird, sandy doughnuts.
Source: NYT Health - Category: Consumer Health News Authors: Tags: Books and Literature Lyme Disease Dunham, Lena your-feed-selfcare Ehlers-Danlos Syndrome Endometriosis Source Type: news
Condition:   Lyme Borreliosis Interventions:   Diagnostic Test: DualDur dark-field microscopic test;   Diagnostic Test: DualDur dark-field automatic microscopic test;   Diagnostic Test: Western blot IgM and IgG;   Diagnostic Test: Bózsik Western blot IgM and IgG;   Diagnostic Test: Standard Polymerase chain reaction;   Dia gnostic Test: enzyme-linked immunosorbent assay (ELISA) IgM and IgG;   Diagnostic Test: DualDur Polymerase chain reaction Sponsors:   Lyme Diagnostics Ltd.;   Pharmahun...
Source: ClinicalTrials.gov - Category: Research Source Type: clinical trials
(Natural News) If you spend any amount of time outdoors or around animals, you’re at risk of Lyme disease. This infectious disease is transmitted by ticks, but it’s actually caused by a microbe, the Borrelia burgdorferi sensu lato bacteria. While many prevention methods focus on the ticks themselves, some researchers are looking into antimicrobial herbs...
Source: NaturalNews.com - Category: Consumer Health News Source Type: news
This article is protected by copyright. All rights reserved. PMID: 30854633 [PubMed - as supplied by publisher]
Source: European Journal of Immunology - Category: Allergy & Immunology Authors: Tags: Eur J Immunol Source Type: research
More News: Epidemiology | Genetics | Infectious Diseases | Laboratory Medicine | Lyme Disease | Microbiology