Virulence-associated genes and antimicrobial resistance among avian pathogenic Escherichia coli from colibacillosis affected broilers in Pakistan

In this study, we determined the VAGs and antibiotic resistance profiles of APEC isolates recovered from colibacillosis affected broilers in Faisalabad region of Pakistan. A total of 84 diseased and dead birds from different local broilers farms were collected and examined for the gross lesions of colibacillosis by conducting postmortem examination. Of these, APEC isolates were recovered from 75 (89.2%) birds. Antibiotic susceptibility tests against 11 antimicrobial agents showed the highest resistance against ampicillin (98.6%) followed by tetracycline (97.3%) and ciprofloxacin (72%). The presence of 11 virulence-associated genes (VAGs) was detected by multiplex polymerase chain reaction (PCR). Of the 75 APEC, 32 (42.6%) harbored> 5 VAGs. Most commonly found genes were increased serum survival (iss; 84%), iron transport (iutA; 74.6%), and colicin V (ColV; 60%). Twenty-two isolates (29.3%) were found to possess a combination of VAGs;iss,tsh,iroN, andiutA, in addition to other VAGs. To the best of our knowledge, this is the first report on the detection of virulence-associated genes and multidrug resistance among APEC isolates in Pakistan. In the future, the strains with the predominant set of VAGs can be used for colibacillosis diagnosis and as a potential vaccine candidate.
Source: Tropical Animal Health and Production - Category: Veterinary Research Source Type: research

Related Links:

The objective of this work was to evaluate the physicochemical characteristics and technological functional properties of okara flours (OFs) obtained by different drying processes in forced air oven (FAD at 40, 50, 60, and 70 °C), microwave (MWD), and freeze drying (FRD). The OFs showed acidity range of 3–5.7 mEq NaOH/100 g, pH of 5.4–5.9, soluble solid of 0.6–1.3 ºBrix, luminosity of 78–85, and good technological functional properties (MWD-OF and FRD-OF showed ↑oil absorption capacity and ↑emulsifying capacity and FRD-OF showed foam capacity). The FAD70-OF, MWD-OF, and FRD-OF...
Source: LWT Food Science and Technology - Category: Food Science Source Type: research
Publication date: Available online 20 January 2020Source: Ticks and Tick-borne DiseasesAuthor(s): Luís Fernando Parizi, Carolina Konrdörfer Rangel, Gabriela Alves Sabadin, Bianca Fagundes Saggin, Irene Kiio, Marina Amaral Xavier, Renata da Silva Matos, Maria Izabel Camargo-Mathias, Adriana Seixas, Satoru Konnai, Kazuhiko Ohashi, Naftaly Wang'ombe Githaka, Itabajara da Silva VazAbstractRhipicephalus appendiculatus, the brown ear tick, is an important disease vector of livestock in eastern, central and southern Africa. Rhipicephalus appendiculatus acaricide resistance requires the search for alternative methods f...
Source: Ticks and Tick borne Diseases - Category: Zoology Source Type: research
This study assessed whether the endemic status of this disease was stable or instable, which is important for disease control efforts. For this aim, 4115 sheep blood samples were collected from 81 cities in the seven geographical regions of Turkey. The diagnosis of Babesia ovis was made using microscopic and serological techniques. Thin blood smears were prepared from anticoagulated venous blood. Serum samples were screened for specific antibodies using an enzyme-linked immunosorbent assay (ELISA) and indirect fluorescent antibody test (IFAT). Recombinant Babesia ovis secreted antigen 1 (rBoSA1) was used in the ELISA. The ...
Source: Veterinary Parasitology - Category: Veterinary Research Source Type: research
Publication date: 15 April 2020Source: Journal of Molecular Structure, Volume 1206Author(s): Anna ZawadaAbstractWith the help of Mössbauer investigations, it was found that tetrahedral coordination is present in the alkali silicate glass system for both Fe2+ and Fe3+ in oxidising molten glass. The reduction process changes the coordination number of iron from 4 to 6. It is clear that the iron content has a much stronger influence on the properties than the redox state. In lower temperatures, the difference between reducing and oxidising conditions can be clearly observed, which at high temperatures lies within the mea...
Source: Journal of Molecular Structure - Category: Molecular Biology Source Type: research
Abstract Campylobacter jejuni and C. coli are leading causes of human foodborne illness with poultry as a major vehicle. Turkeys are frequently colonized with Campylobacter but little is known about Campylobacter survival in turkey feces, even though fecal droppings are major vehicles for Campylobacter within-flock transmission as well as for environmental dissemination. Our objective was to examine survival of Campylobacter, including different strains, in freshly-excreted feces from naturally-colonized commercial turkey flocks and in suspensions of turkey feces in water from the turkey house. Fecal and water sus...
Source: Applied and Environmental Microbiology - Category: Microbiology Authors: Tags: Appl Environ Microbiol Source Type: research
Avian pathogenic Escherichia coli (APEC) is the principle cause of colibacillosis affecting poultry. The main challenge to the poultry industry is antimicrobial resistance and the emergence of multidrug resistant...
Source: BMC Veterinary Research - Category: Veterinary Research Authors: Tags: Research article Source Type: research
This study revealed that a diverse group of plasmids, carrying AR genes, are responsible for the phenotypic resistance seen in Salmonella isolated from United States food animals. It was also determined that many plasmids carry similar ARCs. Introduction Non-typhoidal Salmonella enterica is one of the most common causes of foodborne illnesses globally, with an estimated 1.2 million cases each year in the United States alone (CDC, 2013). Symptoms range from self-limiting gastrointestinal illness to sepsis. These infections can lead to death unless treated with antibiotics (Crump et al., 2015). Unfortunately, antimic...
Source: Frontiers in Microbiology - Category: Microbiology Source Type: research
This report describes the dynamics of FQ-associated mutations in the highly resistant in FQ mutants in S. Enteritidis. In addition, we characterized a deletion in the ramRA integenic region, demonstrating that this frequent mutation in the highly resistant FQ mutants provide resistance or reduce susceptibility to multiple families of antibiotics. Introduction Non-typhoidal Salmonella (NTS) is a major zoonotic pathogen worldwide (Bangtrakulnonth et al., 2004; Scallan et al., 2011). Infections caused by this pathogen have been mainly associated with gastroenteritis, an acute self-limiting intestinal infection. Howeve...
Source: Frontiers in Microbiology - Category: Microbiology Source Type: research
This study highlights the public health importance of enhanced antimicrobial resistance surveillance and strict antimicrobial stewardship in human and veterinary healthcare. PMID: 30475200 [PubMed - as supplied by publisher]
Source: Journal of Medical Microbiology - Category: Microbiology Authors: Tags: J Med Microbiol Source Type: research
Author Affiliations open 1Center for Disease Dynamics, Economics &Policy, Washington, DC, USA 2Public Health Foundation of India, New Delhi, Delhi, India 3Dept. of Veterinary Population Medicine, University of Minnesota, St. Paul, Minnesota, USA 4School of Public Health and Zoonoses, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, Punjab, India 5Dept. of Ecology and Evolutionary Biology, Princeton University, Princeton, New Jersey, USA 6SRL Diagnostics, FORTIS Hospital, Noida, Uttar Pradesh, India 7SRL Diagnostics, Mumbai, Maharashtra, India 8Veterinary &Animal Husbandry Extension, Guru Angad D...
Source: EHP Research - Category: Environmental Health Authors: Tags: Research Source Type: research
More News: Antimicrobial Resistance | Bird Flu | Cipro | Ciprofloxacin | Gastroenteritis | Genetics | Iron | Multidrug Resistance | Pakistan Health | Study | Tetracycline | Vaccines | Veterinary Research | Veterinary Vaccinations