Human testis phosphoproteome reveals kinases as potential targets in spermatogenesis and testicular cancer.

Human testis phosphoproteome reveals kinases as potential targets in spermatogenesis and testicular cancer. Mol Cell Proteomics. 2019 Jan 25;: Authors: Castillo J, Knol JC, Korver CM, Piersma SR, Pham TV, Goeij de Haas RR, van Pelt AMM, Jimenez CR, Jansen BJH Abstract Spermatogenesis is a complex cell differentiation process that includes marked genetic, cellular, functional and structural changes. It requires tight regulation, since disturbances in any of the spermatogenic processes would lead to fertility deficiencies as well as disorders in offspring. In order to increase our knowledge of signal transduction during sperm development, we carried out a large-scale identification of the phosphorylation events that occur in the human male gonad. Metal oxide affinity chromatography using TiO2combined with LC-MS/MS was conducted to profile the phosphoproteome of adult human testes with full spermatogenesis. A total of 8187 phosphopeptides derived from 2661 proteins were identified, resulting in the most complete report of human testicular phosphoproteins to date. Phosphorylation events were enriched in proteins functionally related to spermatogenesis, as well as to highly active processes in the male gonad, such as transcriptional and translational regulation, cytoskeleton organization, DNA packaging, cell cycle and apoptosis. Moreover, 174 phosphorylated kinases were identified. The most active human protein kinases in the testis were ...
Source: Molecular and Cellular Proteomics : MCP - Category: Molecular Biology Authors: Tags: Mol Cell Proteomics Source Type: research