[Characterization of Klebsiella pneumoniae strains exposed to subinhibitory concentrations of chlorhexidine].

[Characterization of Klebsiella pneumoniae strains exposed to subinhibitory concentrations of chlorhexidine]. Mikrobiyol Bul. 2019 Jan;53(1):114-117 Authors: Duman GG, Çuhadar T, Yamak AS, Albakkour K, Çağlar K, Kalkancı A Abstract Chlorhexidine, a topical antiseptic, acts as a cationic biguanide altering the osmotic transport of the bacterial cell wall that has been used throughout the world to prevent healthcare-associated infections for decades. The routine application of chlorhexidine can result in decreased susceptibility of bacteria over time. The aim of this study was to develop Klebsiella pneumoniae strains after exposure to chlorhexidine and characterize these adapted strains in terms of their virulence ability both by in vivo and in vitro methods. Two clinical strains of K.pneumoniae were included in the study. One strain was completely susceptible and the other was resistant to certain antibiotics. Susceptible strain was subjected in the exposure assay as parent/wild strain. Exposure was performed by increasing chlorhexidine concentrations in agar plates. Chlorhexidine concentrations were gradually decreased reaching a final concentration of 0.12 mg/L after five weeks. Chlorhexidine-adapted viable colonies were selected and isolated. Minimal inhibitor concentrations of chlorhexidine, sodium hypochloride, benzalkonium chloride and triclosan for K.pneumoniae strains were determined using broth microdilution method. Rever...
Source: Mikrobiyoloji Bulteni - Category: Microbiology Tags: Mikrobiyol Bul Source Type: research