GSE125676 Two distinct interstitial macrophage populations coexist across tissues in unique subtissular niches [lung interstitial]

Contributors : Svetoslav Chakarov ; Hwee Y Lim ; Leonard Tan ; Sheau Y Lim ; Peter See ; Josephine Lum ; Xiaomeng Zhang ; Shihui Foo ; Satoshi Nakamizo ; Duan Kaibo ; Wan T Kong ; Rebecca Gentek ; Akhila Balachander ; Daniel Carbajo ; Camille Bleriot ; Benoit Malleret ; John K Tam ; Sonia Baig ; Muhammad Shabeer ; Sue-Anne E Toh ; Andreas Schlitzer ; Anis Larbi ; Thomas Marichal ; Bernard Malissen ; Jinmiao Chen ; Michael Poidinger ; Kenji Kabashima ; Marc Bajenoff ; Lai G Ng ; Veronique Angeli ; Florent GinhouxSeries Type : Expression profiling by high throughput sequencingOrganism : Mus musculusMacrophages are a heterogeneous cell population involved in tissue homeostasis, inflammation and in multiple pathologies. Although the major tissue-resident macrophage populations have been extensively studied, interstitial macrophages (IMs) residing within tissue parenchyma remain poorly defined. Here, we studied IMs from murine lung, fat, heart and dermis. We identified two independent IM subpopulations that are conserved across tissues: Lyve1loMHCIIhiCX3CR1hi (Lyve1loMHCIIhi) and Lyve1hiMHCIIloCX3CR1lo (Lyve1hiMHCIIlo) monocyte-derived IMs, with distinct gene expression profiles, phenotypes, functions, and localisation. Using a mouse model of inducible macrophage depletion (SLCO2B1-DTR), we found that the absence of Lyve1hiMHCIIlo IMs exacerbated experimental lung fibrosis. Thus, we demonstrate that two independent populations of IMs exist across tissues a...

Source: GEO: Gene Expression Omnibus - January 26, 2019 Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Mus musculus Source Type: research