Kidney nanotoxicity studied in human renal proximal tubule epithelial cell line TH1

Publication date: Available online 24 January 2019Source: Mutation Research/Genetic Toxicology and Environmental MutagenesisAuthor(s): Monika Sramkova, Katarina Kozics, Vlasta Masanova, Iveta Uhnakova, Filip Razga, Veronika Nemethova, Petra Mazancova, Lucyna Kapka-Skrzypczak, Marcin Kruszewski, Marta Novotova, Victor F. Puntes, Alena GabelovaAbstractProgressive expansion of nanomaterials in our everyday life raises concerns about their safety for human health. Although kidneys are the primary organs of xenobiotic elimination, little attention has been paid to the kidneys in terms of nanotoxicological studies up to now. Here we investigate the cytotoxic and genotoxic potential of four solid-core uncoated inorganic nanoparticles (TiO2NPs, SiO2NPs, Fe3O4NPs and AuNPs) using the human renal proximal tubule epithelial TH1 cells. To mimic the in vivo conditions more realistic, TH1 cells were exposed in vitro to inorganic NPs under static as well as dynamic conditions for 3 h and 24 h. The medium throughput alkaline comet assay (12 minigels per slide) was employed to evaluate the impact of these NPs on genome integrity and their capacity to produce oxidative lesions to DNA. The accumulation and localization of studied inorganic NPs inside the cells was monitored by transmission electron microscopy (TEM) and the efficacy of internalization of particular NPs was determined by atomic absorption spectroscopy (AAS) and inductively coupled plasma mass spectrometry (ICP-MS). From all...
Source: Mutation Research Genetic Toxicology and Environmental Mutagenesis - Category: Genetics & Stem Cells Source Type: research