Effects of the culture medium and the methodology applied on the biofilm formation of 2diarrheagenic Escherichia coli strains.

[Effects of the culture medium and the methodology applied on the biofilm formation of 2diarrheagenic Escherichia coli strains]. Rev Argent Microbiol. 2019 Jan 18;: Authors: Cáceres ME, Etcheverría AI, Padola NL Abstract The ability to form biofilms of pathogenic microorganisms in a wide variety of environments, surfaces and conditions constitute an important risk, both for the food industry and for public health. The aim of this work was to evaluate and to compare the effects of the methodology applied and the culture medium used on the ability of a non-O157 verotoxigenic Escherichia coli strain and an enteropathogenic strain to form biofilm on polystyrene surface. Two methodological variants were tested in static culture and culture mediums with different composition were used. The results showed that both strains were able to form a greater biofilm under culture in LB supplemented with glucose, with medium replacement at 24h and the quantification of the biofilm carried out at 48h of incubation. These conditions could be used in future studies on biofilm formation. PMID: 30665746 [PubMed - as supplied by publisher]
Source: Revista Argentina de Microbiologia - Category: Microbiology Tags: Rev Argent Microbiol Source Type: research

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Authors: Nekrasov KA, Vikarchuk MV, Rudenko EE, Ivanitskiy IV, Grygorenko VM, Danylets RO, Kondratov AG, Stoliar LA, Sharopov BR, Kashuba VI Abstract The detection of prostate cancer (PCa) biomarkers in bodily fluids, a process known as liquid biopsy, is a promising approach and particularly beneficial when performed in urine samples due to their maximal non-invasiveness requirement of collection. A number of gene panels proposed for this purpose have allowed discrimination between disease-free prostate and PCa; however, they bear no significant prognostic value. With the purpose to develop a gene panel for PCa dia...
Source: Oncology Letters - Category: Cancer & Oncology Tags: Oncol Lett Source Type: research
In conclusion, Abdominal DTs were prone to occur in patients with FAP with a history of abdominal surgery. Mutations in the APC, CTNNB1 and MUTYH genes were detected in patients with DTs. To the best of our knowledge, this is the first study of abdominal DT occurrence in patients with MUTYH-associated FAP. The prognosis of DT associated with FAP may be worse compared with that of sporadic DT. PMID: 31807167 [PubMed]
Source: Oncology Letters - Category: Cancer & Oncology Tags: Oncol Lett Source Type: research
In conclusion, the results suggest that, because of its bioactivity including the antioxidant, antimicrobial, and enzyme inhibition properties, the D. macrolepis can be accepted as a promising and natural source for the industrial applications. The present study is the first study, in which the bioactive components and the antioxidant, antimicrobial, and enzyme inhibition properties of endemic D. macrolepis plant were determined.
Source: Saudi Pharmaceutical Journal - Category: Drugs & Pharmacology Source Type: research
ConclusionThis study demonstrates that ESAT6 bound with TLR2 can activate iNOS/NO and ROS signalings to reduce the trimethylation of H3K27 resulting in the increment of EMMMs expression that is beneficial to the transition of macrophages into epithelioid macrophages. However, hypoxia can inhibit this transition event. This study has provided new evidence of pathogenesis of granuloma caused by Mtb and also proposed new ideas for the treatment of TB.
Source: Molecular Immunology - Category: Allergy & Immunology Source Type: research
CONCLUSION: Enterococcus spp. with susceptibility to limited antibiotics was one of the main pathogens in Korean IAIs, along with E. coli and Klebsiella spp., which were highly susceptible to imipenem, amikacin, and piperacillin/tazobactam. Meanwhile, the low susceptibilities of E. coli or Klebsiella spp. to amoxicillin/clavulanate, advanced-generation cephalosporins, and ciprofloxacin should be considered when determining empirical antibiotic therapy in clinical practice. PMID: 31808326 [PubMed - in process]
Source: Journal of Korean Medical Science - Category: Biomedical Science Tags: J Korean Med Sci Source Type: research
Publication date: Available online 6 December 2019Source: Food Research InternationalAuthor(s): Liping Guo, Yongcai Sun, Yinglian Zhu, Baowei Wang, Lin Xu, Ming Huang, Yufeng Li, Jingxin SunAbstractIn the present study, the action mechanism of ultrasound (US) combined with sodium hypochlorite (SH) against Escherichia coli was illustrated by different analysis, including reduction, particle size distribution, scanning electron microscopy (SEM), transmission electron microscopy (TEM), K+ leakage, confocal laser scanning microscopy (CLSM) and fluorescence spectroscopy of Escherichia coli. The results showed that ultrasound im...
Source: Food Research International - Category: Food Science Source Type: research
Publication date: 15 February 2020Source: Journal of Molecular Structure, Volume 1202Author(s): Satyendra Nath Shukla, Pratiksha Gaur, Mohan Lal Raidas, Bhaskar ChaurasiaAbstractTwo Schiff base ligands namely bis-2-((1E)-((Z)-2-(1,2-diphenylethylideneamino) phenylimino)methyl)phenol (L1) and bis-2-((1E)-((E)-2-(1-phenylethylideneamino)phenylimino) methyl)phenol (L2) were synthesized by tailored reaction of o-phenylenediamine with o-hydroxybenzaldehyde and 2-hydroxy-1,2-diphenylethanone/o-hydroxyacetophenone. The reaction of these ligands with Fe(III), Co(II), and Ni(II) salts yielded six complexes. Ligands and their metal ...
Source: Journal of Molecular Structure - Category: Molecular Biology Source Type: research
Publication date: Available online 6 December 2019Source: Catalysis TodayAuthor(s): Linghui Li, Yan Li, Yanzhang Li, Anhuai Lu, Hongrui Ding, Po Keung Wong, Hongli Sun, Junxian ShiAbstractTungstate oxide is a new kind of anionic semiconductor material, which has been received much attention due to its good photocatalytic performance and long-term stability. To facilitate large-scale application, the natural counterpart of tungstate oxide, wolframite, was firstly used as a visible-light mineral photocatalyst in this study. Its mineralogical and photocatalytic properties were characterized and compared with two synthetic end...
Source: Catalysis Today - Category: Chemistry Source Type: research
Publication date: Available online 6 December 2019Source: Journal of Photochemistry and Photobiology B: BiologyAuthor(s): Sai Kumar Tammina, Yang Wan, Yan Yan Li, Yaling YangAbstractA simple and one-step microwave digestion method was utilized to synthesize the highly photoluminescent glucosamine derived nitrogen and zinc doped carbon dots (N, Zn-CDs) with a bluish-green luminescence property. The synthesized N, Zn-CDs inherited a good photoluminescence property with a quantum yield of 74% and which were characterized with XPS, XRD, TEM, FT-IR, UV–Vis, and fluorescence spectrometry. More importantly N, Zn-CDs served ...
Source: Journal of Photochemistry and Photobiology B: Biology - Category: Speech-Language Pathology Source Type: research
Publication date: Available online 5 December 2019Source: Materials LettersAuthor(s): Sheena Raina, Anupma Thakur, Aditi Sharma, D. Pooja, Anu Priya MinhasAbstractThe present was the first report on the synthesis of Carbon dots (CDs) using the acetone leaf extract of Cannabis sativa, an abundant weed plant as a sole carbon precursor. The obtained ∼5 nm spherical CD particles (exhibiting 13% absolute quantum yield) were further to synthesize silver@CDs (Ag@CDs). CDs and Ag@CDs characterization revealed characteristic functional groups attributing to the aromatic, carboxylated and hydroxylated moieties. Ether and acetone...
Source: Materials Letters - Category: Materials Science Source Type: research
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