An insight into bacterial phospholipase C classification and their translocation through Tat and Sec pathways: A data mining study

This study documents a sequence-based method for the classification of putative PLCs from different bacterial sources as well as prediction of their translocation pathways. The analysis indicates that PLCs belonging to a particular group show a distinct conservation of signature residues based on which classification could be done. Tat- and Sec-directed PLCs show differences in the characteristics of their signal peptide which include presence or absence of twin-arginine motif, signal peptide length and hydrophobicity. These features were used as markers for the assignment of translocation pathways. Majority of the PLCs were recognized as members of acid phosphatase superfamily and mostly show a tendency towards Tat pathway. Furthermore, this study confirms that apart from Sec-directed proteins, Tat-directed proteins also possess SecB-binding site implying the non-existence of any sequence-level distinction between Sec- and Tat-specific PLCs with respect to the number of potential SecB-binding site(s).

Source: Meta Gene - January 24, 2019 Category: Genetics & Stem Cells Source Type: research