Early blastocyst expansion in euploid and aneuploid human embryos: evidence for a non-invasive and quantitative marker for embryo selection

Publication date: Available online 23 January 2019Source: Reproductive BioMedicine OnlineAuthor(s): TTF Huang, DH Huang, HJ Ahn, C Arnette, CTF HuangAbstractResearch QuestionHow can the kinetics of human blastocyst expansion be used to evaluate an embryo's ploidy identified using PGT-A?DesignThis was a retrospective observational study of 188 autologous blastocysts from 34 sequential treatment cycles using PGT-A and blastocyst biopsy. Using time lapse imaging, blastocyst expansion was evaluated using a quantitative, standardized expansion assay (qSEA). Trophectoderm cell division was examined in selected, unbiopsied embryos to evaluate the contribution of mitosis to expansion rate.ResultsThe averaged euploid blastocyst expansion rate was significantly faster than in aneuploid blastocysts by 52.8% (p = 0.0041). Scatterplots, representing “expansion maps,” revealed that both populations showed a similarly overlapping distribution of blastocyst formation times during 80-140 hrs from fertilization. Euploidy and aneuploidy was better distinguished in regions of higher or lower expansion, respectively, in expansion maps. Based upon expansion slopes, rank ordering of individual embryos within cohorts resulted in euploidy enrichment of>90% within the first two ranks in patients
Source: Reproductive BioMedicine Online - Category: Reproduction Medicine Source Type: research

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