IDO, COX and iNOS have an important role in the proliferation of Neospora caninum in neuron/glia co-cultures

Publication date: Available online 22 January 2019Source: Veterinary ParasitologyAuthor(s): L.B Jesus, A.B. Santos, E.E.V. Jesus, R.G.D. Santos, M.S. Grangeiro, A.B. Silva, M.R. Arruda, D.S. Argolo, A.M Pinheiro, R.S. El- Bachá, S.L. Costa, M.F.D. CostaAbstractCentral nervous system (CNS) is the main site for encystment of Neospora caninum in different animal species. In this tissue, glial cells (astrocytes and microglia) modulate responses to aggression in order to preserve homeostasis and neuronal function. Previous data showed that when primary cultures of glial cells are infected with N. caninum, they develop gliosis and the immune response is characterized by the release of TNF and IL-10, followed by the control of parasite proliferation. In order to elucidate this control, three enzymatic systems involved in parasite-versus-host interactions were observed on a model of neuron/glia co/cultures obtained from rat brains. Indoleamine 2,3-dioxygenase (IDO), induced nitric oxide synthase (iNOS) responsible for the catabolism of tryptophan and arginine, respectively, and cycloxigenase (COX) were studied comparing their modulation by respective inhibitors with the number of tachyzoites or the immune response measured by the release of IL-10 and TNF. Cells were treated with the inhibitors of iNOS (1.5 mM L-NAME), IDO (1 mM 1-methyl tryptophan), COX-1 (1 μM indomethacin) and COX-2 (1 μM nimesulide) before infection with tachyzoites of N. caninum (1:1 cell: parasite). A...
Source: Veterinary Parasitology - Category: Veterinary Research Source Type: research