Scanning transmission X-ray microscopy with efficient X-ray fluorescence detection (STXM-XRF) for biomedical applications in the soft and tender energy range

Scanning transmission X-ray microscopy, especially in combination with X-ray fluorescence detection (STXM-XRF) in the soft X-ray energy range, is becoming an increasingly important tool for life sciences. Using X-ray fluorescence detection, the study of biochemical mechanisms becomes accessible. As biological matrices generally have a low fluorescence yield and thus a low fluorescence signal, high detector efficiency (e.g. large solid angle) is indispensable for avoiding long measurement times and radiation damage. Here, the new AnImaX STXM-XRF microscope equipped with a large solid angle of detection enabling fast scans and the first proof-of-principle measurements on biomedical samples are described. In addition, characterization measurements for future quantitative elemental imaging are presented.

Source: Journal of Synchrotron Radiation - January 21, 2019 Category: Physics Authors: L ü hl, L. Andrianov, K. Dierks, H. Haidl, A. Dehlinger, A. Heine, M. Heeren, J. Nisius, T. Wilhein, T. Kanngie ß er, B. Tags: X-ray microscopy X-ray fluorescence biomedical applications research papers Source Type: research