Biofilm ‐induced profiles of immune response gene expression by oral epithelial cells

This study examined the oral epithelial immunotranscriptome response patterns modulated by oral bacterial planktonic or biofilm challenge. We assessed gene expression patterns when epithelial cells were challenged with a multispecies biofilm composed ofStreptococcus gordonii, Fusobacterium nucleatum, andPorphyromonas gingivalisrepresenting a type of periodontopathic biofilm compared to challenge with the same species of planktonic bacteria. Of the 579 human immunology genes, a substantial signal of the epithelial cells was observed to 181 genes. Biofilm challenged stimulated significant elevations compared to planktonic bacteria for IL32, IL8, CD44, B2M, TGFBI, NFKBIA, IL1B, CD59, IL1A, CCL20 representing the top 10 signals comprising 55% of the overall signal for the epithelial cell responses. Levels of PLAU, CD9, IFITM1, PLAUR, CD24, TNFSF10, and IL1RN were all elevated by each of the planktonic bacterial challenge vs the biofilm responses. While the biofilms up ‐regulated 123/579 genes (>2 ‐fold), fewer genes were increased by the planktonic species (36 [S gordonii], 30 [F nucleatum], 44 [P gingivalis]). A wide array of immune genes were regulated by oral bacterial challenge of epithelial cells that would be linked to the local activity of innate and adaptive immune response components in the gingival tissues. Incorporating bacterial species into a structured biofilm dramatically altered the number and level of genes expressed. Additionally, a specific set of genes ...
Source: Molecular Oral Microbiology - Category: Microbiology Authors: Tags: ORIGINAL ARTICLE Source Type: research

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Publication date: December 2019Source: The Journal of Prosthetic Dentistry, Volume 122, Issue 6Author(s): Maria Sílvia Maurício Rigolin, Paula Aboud Barbugli, Janaina Habib Jorge, Marina Rosa Delgado Reis, Gelson Luis Adabo, Luciana Assirati Casemiro, Carlos Henrique Gomes Martins, Omar José de Lima, Francisco de Assis Mollo JuniorAbstractStatement of problemThe longevity of dental implants depends on the maintenance of peri-implant tissue and absence of inflammation. How the physical-chemical properties intrinsic to each material over time can affect adhesion, given constant cell turnover and biofilm ...
Source: The Journal of Prosthetic Dentistry - Category: Dentistry Source Type: research
DNA and Cell Biology, Ahead of Print.
Source: DNA and Cell Biology - Category: Cytology Authors: Source Type: research
This report describes a rare case of acute pylephlebitis complicated by hepatic abscess formation secondary to Fusobacterium nucleatum. The patient is a 48-year-old woman who presented with worsening abdominal pain and malaise for 3 weeks. She had a Roux-en-Y gastric bypass surgery 4 months earlier that showed a phlegmon at the surgical site on a contrast computed tomography scan postsurgery. Physical examination revealed right upper quadrant tenderness, and magnetic resonance imaging of the liver showed portal vein thrombosis with multiple hepatic abscesses. The patient was started empirically on cefepime and metronidazol...
Source: Infectious Diseases in Clinical Practice - Category: Infectious Diseases Tags: Case Reports Source Type: research
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Source: Frontiers in Genetics - Category: Genetics & Stem Cells Source Type: research
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Source: European Respiratory Journal - Category: Respiratory Medicine Authors: Tags: Idiopathic interstitial pneumonias Source Type: research
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Source: European Respiratory Journal - Category: Respiratory Medicine Authors: Tags: Paediatric respiratory infection and immun. Source Type: research
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Source: Advances in Experimental Medicine and Biology - Category: Research Tags: Adv Exp Med Biol Source Type: research
In this study, we analyzed and compared the intestinal microbiota composition of patients with idiopathic focal epilepsy (n = 30) and healthy volunteer group (n = 10) by 16s ribosomal DNA sequencing. Proteobacteria phylum was found to be higher in patients with epilepsy (25.4%) than in healthy volunteers group (1.5%). The genera of Campylobacter, Delftia, Haemophilus, Lautropia, Neisseria among Proteobacteria phylum were found to be statistically significantly higher in patients with epilepsy than in healthy volunteers (p 
Source: Microbial Pathogenesis - Category: Infectious Diseases Source Type: research
ConclusionsThis study shows that the alveolar microbiota profile in BAL samples from TB and IP patients, representing infectious and non-infectious lung diseases, respectively, is characterized by decreased diversity.
Source: Microbial Pathogenesis - Category: Infectious Diseases Source Type: research
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Source: Journal of Oral Biosciences - Category: Biomedical Science Source Type: research
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