Chimeric mice with human hepatocytes: a new system for genotoxicity studies

Publication date: Available online 14 January 2019Source: Mutation Research/Genetic Toxicology and Environmental MutagenesisAuthor(s): Chise Tateno, Masahito Fukumuro, Shoji Masumori, Masakazu Kakuni, Yuji Ishida, Takashi Shimada, Makoto HayashiAbstractGenotoxicity assays are characterized by a method, an in vitro or in vivo target, and an endpoint. Many cell types have been used as targets, including bacterial cells, cultured mammalian cells, and rodent cells in vivo. Human cells are the most important target for evaluating the risk to humans associated with exposure to chemicals. Almost exclusively, the human cells used in genotoxicity tests have been cultured cells. Here, we have tested human hepatocytes in PXB-mice®, chimeric mice in which the liver has been repopulated with human hepatocytes, as a source of target cells for in vivo genotoxicity assays. We applied the single-cell gel electrophoresis (comet) assay to detect DNA damage and the micronucleus assay to evaluate chromosomal aberrations. These chimeric mice can serve as a valuable model system for genotoxicity assays.
Source: Mutation Research Genetic Toxicology and Environmental Mutagenesis - Category: Genetics & Stem Cells Source Type: research