Participation of opioid receptors in the cytoprotective effect of chronic normobaric hypoxia.

Participation of opioid receptors in the cytoprotective effect of chronic normobaric hypoxia.
Physiol Res. 2019 Jan 10;
Authors: Naryzhnaya NV, Khaliulin I, Lishmanov YB, Suleiman MS, Tsibulnikov SY, Kolar F, Maslov LN
Abstract
We studied the role of the delta, micro, and kappa opioid receptor (OR) subtypes in the cardioprotective effect of chronic continuous normobaric hypoxia (CNH) in the model of acute anoxia-reoxygenation of isolated cardiomyocytes. Adaptation of rats to CNH was performed by their exposure to atmosphere containing 12% of O(2) for 21 days. Anoxia-reoxygenation of cardiomyocytes isolated from normoxic control rats caused the death of 51 % of cells and lactate dehydrogenase (LDH) release. Adaptation of rats to CNH resulted in the anoxia/reoxygenation-induced cardiomyocyte death of only 38 %, and reduced the LDH release by 25 %. Pre-incubation of the cells with either the non-selective OR (opioid receptor) blocker naloxone (300 nM/l), the delta OR antagonist TIPP(psi) (30 nM/l), the selective delta(2) OR antagonist naltriben (1 nM/l) or the micro OR antagonist CTAP (100 nM/l) for 25 minutes before anoxia abolished the reduction of cell death and LDH release afforded by CNH. The antagonist of delta(1) OR BNTX (1 nM/l) or the kappa OR antagonist nor-binaltorphimine (3 nM/l) did not influence the cytoprotective effects of CNH. Taken together, the cytoprotective effect of CNH is associated with the activation of the delt...

Source: Physiological Research - January 10, 2019 Category: Physiology Authors: Naryzhnaya NV, Khaliulin I, Lishmanov YB, Suleiman MS, Tsibulnikov SY, Kolar F, Maslov LN Tags: Physiol Res Source Type: research