Heat shock protein-70-mediated heat preconditioning attenuates hepatic carbohydrate and oxidative disturbances in rats with type-1 diabetes

Publication date: Available online 11 January 2019Source: Canadian Journal of DiabetesAuthor(s): Katerina Gerazova-Efremova, Suzana Dinevska – Kjovkarovska, Biljana MiovaAbstractHeat preconditioning (HP) and heat shock protein (HSP) synthesis have a significant cytoprotective effect against development of cellular injury caused by application of a subsequent stressor which was found to depend on the time period between the stressors. We aimed to determine the most efficient recovery time (6 and 24 hours) following heat stress exposure and prior application of diabetic streptozotocin (STZ) over moderation of carbohydrate and oxidative metabolic disturbances caused by diabetes. Experimental animals (Wistar rats) were exposed to acute heat stress (AHS) at 41±1ºC for 45 min, followed by a 6 and 24h recovery at room temperature before sacrifice or STZ administration.Our findings indicate that AHS with 6 and 24h recovery results in significant rise of hepatic HSP70 level (even more so after 24h), glycogen breakdown and stable glycemia followed by reduced glycolytic (HK) and gluconeogenic activity (after 24h) (G6Pase, F1,6BPase), stimulated antioxidative activity (GPx, GR) (after 6h) as well as decreased glutathione and Cat activity (after 24h). Heat preconditioning (with 6 and 24h recovery) prior to STZ-diabetes increases HSP70 level, causes lower serum glucose levels, higher glycogen and G6P, lower G6Pase, as well as lower GP and HK, but also elevates GR and GPx activity compa...
Source: Canadian Journal of Diabetes - Category: Endocrinology Source Type: research