Psd2 pea defensin shows a preference for mimetic membrane rafts enriched with glucosylceramide and ergosterol

Publication date: Available online 10 January 2019Source: Biochimica et Biophysica Acta (BBA) - BiomembranesAuthor(s): Virginia Sara Grancieri do Amaral, Caroline Mota Fernandes, Mário R. Felício, Aline Sol Valle, Paula G. Quintana, Caroline Correa de Almeida, Eliana Barreto-Bergter, Sónia Gonçalves, Nuno C. Santos, Eleonora KurtenbachAbstractPsd2 is a pea defensin with 47 amino acid residues that inhibits the growth of fungal species by an uncharacterized mechanism. In this work, Psd2 interactions with model membranes mimicking the lipid compositions of different organisms were evaluated. Protein-lipid overlay assays indicated that Psd2 recognizes Fusarium solani glucosylceramide (GlcCerF.solani) and ergosterol (Erg) in addition to phosphatidylcholine (POPC) and some phosphatidylinositol species, such as PtdIns (3)P, (5)P and (3,5)P2, suggesting that these lipids may play important roles as Psd2 targets. Assays using lipid vesicles were also performed to study the behaviour and dynamics that occur after peptide-membrane interactions. Surface plasmon resonance analysis showed that Psd2 has a higher affinity for pure POPC and POPC-based vesicles containing GlcCer and Erg at a 70:30 proportion than for vesicles containing cholesterol (Chol). Partition experiments by fluorescence spectroscopy showed a decrease in Trp42 quantum yield of Psd2 in the presence of GlcCerF.solani and Erg, individually or in simultaneously enriched membranes. The partition c...
Source: Biochimica et Biophysica Acta (BBA) Biomembranes - Category: Biochemistry Source Type: research

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CONCLUSIONS The anti-AS potential of Rb1 was exerted by restoring the regular function of ECs via the activation of GPER-mediated PI3K/Akt signaling. PMID: 31609302 [PubMed - in process]
Source: Medical Science Monitor - Category: Research Tags: Med Sci Monit Source Type: research
This study was to investigate the degree of susceptibility to intermittent hypoxia (IH), a hallmark of obstructive sleep apnea (OSA), between the two mice inbred lines C57BL/6N (6N) and C57BL/6J (6J).Materials and methodsFour-week old male mice of 6N and 6J substrains (n = 8) were randomized to standard diet (SD) group or high fat (HF) diet group. At the age of 13-week, all two groups of mice were subjected to either air or IH (IH30; thirty hypoxic events per hour) for one week.Key findingsAll mice fed with HF diet exhibited obesity with more body weight and fat mass (percentage to body weight) gain. IH reduced serum L...
Source: Life Sciences - Category: Biology Source Type: research
In this study, a fully integrated microfluidic analyzer which can perform multiple blood biochemical analysis is proposed. The finger-prick blood can be directly aspirated into the chip containing diluent and lyophilized reagents, and the following processes, including serum/plasma separation and metering, diluent release and metering, quantified serum/plasma and diluent mixing, the diluted serum/plasma distribution to the reaction chambers, and the biochemical reaction and results measurement, were sequentially and automatically performed on the self-contained microfluidic chip. Using this one key operated portable analyz...
Source: Sensors and Actuators B: Chemical - Category: Chemistry Source Type: research
Credit: Busani Bafana/IPSBy Esther Ngumbi and Ifeanyi NsoforILLINOIS, United States / ABUJA, Oct 16 2019 (IPS) United Nations World Food Day is celebrated around the world on October 16 under the theme: “Our Actions ARE Our Future. Healthy Diets for a Zero Hunger World”. This theme is timely, especially, because across Africa and around the world, there has been a gradual rise in malnutrition and diet-related non communicable diseases, as highlighted in The Lancet study and a United Nations Report published earlier this year. While 45 percent of deaths in children are from nu...
Source: IPS Inter Press Service - Health - Category: International Medicine & Public Health Authors: Tags: Food & Agriculture Global Headlines Health World Food Day Source Type: news
Journal of Agricultural and Food ChemistryDOI: 10.1021/acs.jafc.9b03731
Source: Journal of Agricultural and Food Chemistry - Category: Food Science Authors: Source Type: research
Publication date: Available online 14 October 2019Source: Life SciencesAuthor(s): Alanna Fernandes Paraíso, Jaciara Neves Sousa, João Marcus Oliveira Andrade, Eloá Santos Mangabeira, Deborah de Farias Lelis, Alfredo Mauricio Batista de Paula, Andréia Maria Eleutério Barros-Lima, William James Nogueira Lima, André Luiz Sena Guimarães, Geraldo Aclécio Melo, Michaela Schwarz, Sérgio Henrique Sousa SantosAbstractAimsThe aim of the presente study was to examine the effects of oral gallic acid (GA) administration on the brown adipose tissue of obese mice fed with hig...
Source: Life Sciences - Category: Biology Source Type: research
Publication date: Available online 15 October 2019Source: Journal of Bioscience and BioengineeringAuthor(s): Adinda P. Wisman, Yoshihiro Tamada, Shuji Hirohata, Katsuya Gomi, Eiichiro Fukusaki, Shuichi ShimmaIn sake brewing, the quality of rice koji is evaluated by experienced sake brewers based on visual inspection of the haze, which is defined by the extent of fungal hyphae spread on/into the rice grains. There is an increasing interest in understanding the factors that affect the quality of rice koji, which is dependent on its making process. Several studies have focused on the degree of mycelial penetration (haze-komi)...
Source: Journal of Bioscience and Bioengineering - Category: Biomedical Science Source Type: research
This appeared last week:Patient portal usage associated with better preventive health engagement By Greg SlabodkinOctober 08, 2019, 11:06 p.m. EDT Patient use of an online portal has been linked to significant improvements in preventive health behaviors, according to a study of 10,000 patients by Penn Medicine researchers. Results of their retrospective observational cohort, published in the Journal of Medical Internet Research, show that those who used an online patient portal were 50 percent more likely to get a flu shot and twice as likely to have their blood pressure checked, compared with patients who do not use ...
Source: Australian Health Information Technology - Category: Information Technology Authors: Source Type: blogs
Evaluating stress levels over time and intervening when high perceived stress persists might reduce hypertension risk Related items fromOnMedica Should we recognise obesity as a disease? Type 2 diabetes in 10 times more young people than realised Pharmacists could offer high-dose statins direct to patients WHO dietary fat guidance fails to consider crucial evidence Plant-based diet linked to lower risk of heart failure
Source: OnMedica Latest News - Category: UK Health Source Type: news
AbstractGlycerol monolaurate (GML) has been widely used as an effective antibacterial emulsifier in the food industry. A total of 360 44-week-old Hy-Line brown laying hens were randomly distributed into four groups each with six replicates of 15 birds, and fed with corn-soybean-meal-based diets supplemented with 0, 0.15, 0.30, and 0.45 g/kg GML, respectively. Our results showed that 0.15, 0.30, and 0.45 g/kg GML treatments significantly decreased feed conversion ratios (FCRs) by 2.65%, 7.08%, and 3.54%, respectively, and significantly increased the laying rates and average egg weights. For egg quality, GML drastically incr...
Source: Journal of Zhejiang University. Science. B. - Category: Universities & Medical Training Source Type: research
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