A novel monoclonal antibodies based sandwich ELISA for detection of serotype 4 fowl adenovirus.

A novel monoclonal antibodies based sandwich ELISA for detection of serotype 4 fowl adenovirus. Avian Pathol. 2019 Jan 09;:1-19 Authors: Shao H, Wang P, Wang W, Zhang J, Li T, Liang G, Gao W, Qin A, Ye J Abstract As a major causative agent for hepatitis-hydropericardium syndrom (HPS) in chickens, serotype 4 fowl adenovirus (FAdV-4) has caused huge economic loss in poultry industry globally. However, there is no commercial diagnostics for FAdV-4 antigens. To generate a rapid approach for specific detection of FAdV-4, a monoclonal antibodies (mAbs) based sandwich ELISA was developed. In this ELISA, a purified mAb 4A3 and HRP labeled mAb 3C2 specific to the fiber2 of FAdV-4 was used as the capture antibody and detection antibody respectively. Specificity assay revealed the ELISA only reacted with FAdV-4, not with other avian viruses tested. Sensitivity assay showed the limit of detection of the ELISA was 1000 TCID50/ml and 12.5 ng/mL for the FAdV-4 and the purified GST-Fiber2 protein respectively. Moreover, the ELISA could be efficiently applied in detecting the FAdV-4 in the tissue samples from the clinical diseased chicken flock. All these data demonstrated that the ELISA developed here provides a promising tool for rapid and efficient diagnosis of the clinical infection of FAdV-4. PMID: 30621493 [PubMed - as supplied by publisher]
Source: Avian Pathology - Category: Pathology Authors: Tags: Avian Pathol Source Type: research