A novel monoclonal antibodies based sandwich ELISA for detection of serotype 4 fowl adenovirus.

A novel monoclonal antibodies based sandwich ELISA for detection of serotype 4 fowl adenovirus. Avian Pathol. 2019 Jan 09;:1-19 Authors: Shao H, Wang P, Wang W, Zhang J, Li T, Liang G, Gao W, Qin A, Ye J Abstract As a major causative agent for hepatitis-hydropericardium syndrom (HPS) in chickens, serotype 4 fowl adenovirus (FAdV-4) has caused huge economic loss in poultry industry globally. However, there is no commercial diagnostics for FAdV-4 antigens. To generate a rapid approach for specific detection of FAdV-4, a monoclonal antibodies (mAbs) based sandwich ELISA was developed. In this ELISA, a purified mAb 4A3 and HRP labeled mAb 3C2 specific to the fiber2 of FAdV-4 was used as the capture antibody and detection antibody respectively. Specificity assay revealed the ELISA only reacted with FAdV-4, not with other avian viruses tested. Sensitivity assay showed the limit of detection of the ELISA was 1000 TCID50/ml and 12.5 ng/mL for the FAdV-4 and the purified GST-Fiber2 protein respectively. Moreover, the ELISA could be efficiently applied in detecting the FAdV-4 in the tissue samples from the clinical diseased chicken flock. All these data demonstrated that the ELISA developed here provides a promising tool for rapid and efficient diagnosis of the clinical infection of FAdV-4. PMID: 30621493 [PubMed - as supplied by publisher]
Source: Avian Pathology - Category: Pathology Authors: Tags: Avian Pathol Source Type: research

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iang Diao Fowl adenovirus serotype 4 (FAdV-4) is the pathogenic agent of hydropericardium hepatitis syndrome (HHS) in chickens and ducks, which has caused huge economic losses for the Chinese poultry industry since 2015. In order to objectively determine the prevalence and co-infection status of the virus in Shandong province in China, we analyzed a total of 679 clinical cases of chickens and ducks from 36 farms in the province. The results showed that the FAdV-4 infection rate was 65.2% (443/679), and the rate in breeder ducks was almost two-fold higher than that in breeder chickens (68.57% vs. 34.30%). Notably, co-in...
Source: Viruses - Category: Virology Authors: Tags: Communication Source Type: research
This study is the first experimental infection of FAdV-4 in geese, which will provide a basis for further understanding of the disease. PMID: 31155930 [PubMed - as supplied by publisher]
Source: Avian Pathology - Category: Pathology Authors: Tags: Avian Pathol Source Type: research
María Maximina B. Moreno-Altamirano1*, Simon E. Kolstoe2 and Francisco Javier Sánchez-García1* 1Laboratorio de Inmunorregulación, Departamento de Inmunología, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, Mexico City, Mexico 2School of Health Sciences, University of Portsmouth, Portsmouth, United Kingdom Over the last decade, there has been significant advances in the understanding of the cross-talk between metabolism and immune responses. It is now evident that immune cell effector function strongly depends on the metabolic pathway in w...
Source: Frontiers in cellular and infection microbiology - Category: Microbiology Source Type: research
Major viruses, including duck-origin avian influenza virus, duck-origin Newcastle disease virus, novel duck parvovirus, duck hepatitis A virus, duck Tembusu virus, fowl adenovirus, and duck enteritis virus, po...
Source: BMC Veterinary Research - Category: Veterinary Research Authors: Tags: Research article Source Type: research
In conclusion, we demonstrated that the VP3 expression mediated by rAAAV in ducklings could induce protective immunity against DHAV challenge, and this could be a candidate vaccine for the control of duck viral hepatitis. Keywords: avian adeno-associated virus; duck hepatitis A virus; VP3 gene; immunogenicity. PMID: 30879313 [PubMed - in process]
Source: Acta Virologica - Category: Infectious Diseases Authors: Tags: Acta Virol Source Type: research
This report describes a novel siadenovirus in a cockatiel that is highly identical to budgerigar adenovirus 1 and distinct from PsAdV-2 in cockatiels. We report the clinical pathologic, gross, and histopathologic findings in a cockatiel with chronic hepatitis and a novel siadenovirus, PsAdV-5. The sequencing data is presented with a phylogenetic analysis. PMID: 30711577 [PubMed - as supplied by publisher]
Source: Virus Research - Category: Virology Authors: Tags: Virus Res Source Type: research
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Source: Transboundary and Emerging Diseases - Category: Veterinary Research Authors: Tags: Original Article Source Type: research
LITFL • Life in the Fast Lane Medical Blog LITFL • Life in the Fast Lane Medical Blog - Emergency medicine and critical care medical education blog aka Tropical Travel Trouble 009 The diagnosis of HIV is no longer fatal and the term AIDS is becoming less frequent. In many countries, people with HIV are living longer than those with diabetes. This post will hopefully teach the basics of a complex disease and demystify some of the potential diseases you need to consider in those who are severely immunosuppressed. While trying to be comprehensive this post can not be exhaustive (as you can imagine any patient with ...
Source: Life in the Fast Lane - Category: Emergency Medicine Authors: Tags: Clinical Cases Tropical Medicine AIDS art cryptococcoma cryptococcus HIV HIV1 HIV2 PEP PrEP TB toxoplasma tuberculoma Source Type: blogs
Abstract During the period from 2015 to 2017, frequent outbreaks of inclusion body hepatitis (IBH) were observed in chicken broilers and falcons in Saudi Arabia. Fifty samples were collected from both species. The histopathological examination and PCR confirmed the IBH infection in eight samples (5 samples from chickens and 3 samples from falcons). The genomic sequence and phylogenetic analysis based on nucleotide and amino acids sequences of Saudi strains, reference fowl aviadenoviruses (FAdVs) and field viruses available in Genbank revealed that, all investigated FAdVs clustered into FAdV-2 (species D) and FAdV-...
Source: Avian Pathology - Category: Pathology Authors: Tags: Avian Pathol Source Type: research
Abstract In June 2015, an infectious disease with high prevalence causing severe hydropericardium syndrome (HPS) first appeared in duck farms of northeast China. The disease showed high morbidity of 35% and mortality of 15% in a commercial duck farm with 200,000 45-day-old ducks. One strain of hypervirulent fowl adenovirus serotype 4 was identified and designated as HLJDAd15. The whole genome of the duck isolate was sequenced and found to contain the same large deletions as a genotype that has become prevalent in chickens in China recently, indicating that this disease might be transmitted from chickens to ducks. ...
Source: Avian Pathology - Category: Pathology Authors: Tags: Avian Pathol Source Type: research
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