Evaluation of Dermal Tissue Regeneration Using Resveratrol Loaded Fibrous Matrix in a Preclinical Mouse Model of Full-Thickness Ischemic Wound

Publication date: Available online 9 January 2019Source: International Journal of PharmaceuticsAuthor(s): Rajesh Lakhsmanan, Jacob Campbell, Gopi Ukani, André O'Reilly Beringhs, Vaithinathan Selvaraju, Mahesh Thirunavukkarasu, Xiuling Lu, J. Alexander Palesty, Nilanjana MaulikAbstractThe complete loss of dermal tissue due to ischemia is a serious challenge facing clinicians. Frequently, the failure of wound healing is due to ischemic conditions prevailing at the site of damaged tissue. Restoration of lost vasculature at the ischemic site can be achieved by supplementing proangiogenic stimuli through an engineered scaffold mimicking dermal extracellular matrix. Towards this objective, we have developed an electrospun scaffold loaded with the pro-angiogenic molecule resveratrol. The physical and chemical changes in the polymeric scaffold before and after loading of resveratrol were characterized using field emission scanning electron microscopy (FE-SEM), Fourier-transform infrared spectroscopy (FT-IR), differential scanning calorimetry (DSC), thermogravimetric analysis (TGA), coherence scanning interferometry (CSI) and X-ray diffraction (XRD). A sustained release of resveratrol from the scaffold was elucidated by UV-spectrophotometer analysis. The enhancement in cell-matrix interaction was studied using human umbilical vein endothelial cells (HUVECs) seeded on the scaffolds. The biocompatibility analysis of resveratrol loaded scaffolds was evaluated through a subcutaneous impl...
Source: International Journal of Pharmaceutics - Category: Drugs & Pharmacology Source Type: research