Rat Hippocampal Neurons

Used to Study Apoptosis Induced by Brain InsultsAlthough ourprimary human cells are increasingly being used in drug discovery and toxicology assays versusanimal cells, we are always pleased to see the success of the latter in sophisticated studies.This publication references use of oure18 rat hippocampal neurons. These cells continue to beeasy to culture, pure and potent. Chiara Porro, Antonia Cianciulli, Teresa Trotta, Dario Domenico Lofrumento, Rosa Calvello and Maria Antonietta Panaro. (2019).Formyl-methionyl-leucyl-phenylalanine Induces Apoptosis in Murine Neurons: Evidence for NO-Dependent Caspase-9 Activation. Biology 8(1), 4; doi:10.3390/biology8010004.HighlightsFormyl-methionyl-leucyl-phenylalanine (fMLP) may be present in the brain in the course of some infectious diseases of the central nervous system (CNS), although little is known about its role. This investigation was performed to study the effect of fMLP on neuron apoptosis. The results showed that fMLP treatment of primary cultures of neurons was able to induce morphological features of apoptosis in cell cultures, as well as activation of the intrinsic apoptotic pathway, through the upregulation of caspase-9 and caspase-3.Figure. (A) Western blot analysis was performed on membrane-enriched cell extracts (25 µg lysate) of primary neurons. The blots were probed with formyl peptide receptor 2 (FPR2) antibody (Ab) and detected by chemiluminescence. A ~41 kDa band corresponding to FPR2 was observed as compared to t...
Source: Neuromics - Category: Neuroscience Tags: cell culturing protocol E18 Primary Hippocampal Neurons fMLP Formyl-methionyl-leucyl phenylalanine primary Neuronal Cells Source Type: news