Rhein, An Anthraquinone Drug, Suppresses the NLRP3 Inflammasome and Macrophage Activation in Urate Crystal-Induced Gouty Inflammation.

This study aimed to evaluate the anti-inflammatory effects of rhein on gouty arthritis. Rhein within the physiological levels of humans showed no toxicity on the cell viability and differentiation, but significantly decreased the production of IL-1 β , TNF- α and caspase-1 protease in urate crystal-activated macrophages. Compared to medium controls, rhein at the therapeutic concentration (2.5 μ g/mL) effectively inhibited IL-1 β production by 47% ( P=0.002 ). Rhein did not affect the mRNA levels of CASP1, NLRP3 and ASC, but suppressed the protein expression and enzyme activity of caspase-1. Immunofluorescence confocal microscopy further revealed that rhein suppressed the aggregation of ASC speck and inhibited the formation of NLRP3 inflammasome. Rhein of 5 μ g/mL significantly decreased the ASC speck to 36% ( P=0.0011 ), and reduced the NLRP3 aggregates to 37.5% ( P=0.014 ). Our data demonstrate that rhein possesses pharmacological activity to suppress caspase-1 protease activity and IL-1 β production by interfering with the formation of NLRP3 multiprotein complex. These results suggest that rhein has therapeutic potential for treating NLRP3 inflammasome-mediated diseases such as gouty arthritis. PMID: 30612459 [PubMed - as supplied by publisher]
Source: The American Journal of Chinese Medicine - Category: Complementary Medicine Authors: Tags: Am J Chin Med Source Type: research

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Source: Daily Express - Health - Category: Consumer Health News Source Type: news
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Source: Journal of Ethnopharmacology - Category: Drugs & Pharmacology Source Type: research
Conditions:   Rheumatoid Arthritis;   Ankylosing Spondylitis;   Fibromyalgia;   Gout;   Crohn Disease;   Juvenile Idiopathic Arthritis;   Lupus Erythematosus;   Myositis;   Osteoarthritis;   Osteoporosis;   Psoriasis;   Psoriatic Arthritis;   Scleroderma Intervention:   Sponsors:   Global Healthy Living Foundation;   University of Alabama at Birmingham Recruiting
Source: ClinicalTrials.gov - Category: Research Source Type: clinical trials
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Source: Journal of Biological Chemistry - Category: Chemistry Authors: Tags: Methods and Resources Source Type: research
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Source: Knee Surgery, Sports Traumatology, Arthroscopy - Category: Orthopaedics Source Type: research
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Source: Hip International - Category: Orthopaedics Authors: Tags: Hip Int Source Type: research
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Source: Rheumatology International - Category: Rheumatology Source Type: research
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Source: Human Immunology - Category: Allergy & Immunology Authors: Tags: Hum Immunol Source Type: research
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Source: Journal of Clinical Orthopaedics and Trauma - Category: Orthopaedics Source Type: research
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Source: Acta Orthopaedica - Category: Orthopaedics Authors: Tags: Acta Orthop Source Type: research
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