Leptin Receptor as a Potential Target to Inhibit Human Testicular Seminoma Growth.

Leptin Receptor as a Potential Target to Inhibit Human Testicular Seminoma Growth. Am J Pathol. 2019 Jan 02;: Authors: Panza S, Gelsomino L, Malivindi R, Rago V, Barone I, Giordano C, Giordano F, Leggio A, Comandè A, Liguori A, Aquila S, Bonofiglio D, Andò S, Catalano S Abstract Although in the last decades, the adipokine leptin and its own receptor have been considered as significant cancer biomarkers, their potential involvement in human testicular seminoma growth and progression remains unexplored. Here, we demonstrated that the expression of leptin and its receptor was significantly higher in human testicular seminoma compared to normal adult testis. Human seminoma cell line TCam-2 also expressed leptin along with the long and the short isoforms of leptin receptor and in response to leptin treatment, showed an enhanced activation of its downstream effectors. In line with these results, leptin stimulation significantly increased proliferation and migration of TCam-2 cells. Treatment of TCam-2 cells with the peptide LDFI, a full leptin receptor antagonist, completely reversed the leptin-mediated effects on cell growth and motility as well as reduced the expression of several leptin-induced target genes. More importantly, the in vivo xenograft experiments revealed that LDFI treatment markedly decreased seminoma tumor growth. Interestingly, LDFI-treated tumors exhibited reduced levels of the proliferation marker Ki-67 as well as a ...
Source: The American Journal of Pathology - Category: Pathology Authors: Tags: Am J Pathol Source Type: research