Rapid and simple universal Escherichia coli genotyping method based on Multiple-Locus Variable-Number of Tandem Repeats Analysis using single-tube multiplex PCR and standard gel electrophoresis.

Rapid and simple universal Escherichia coli genotyping method based on Multiple-Locus Variable-Number of Tandem Repeats Analysis using single-tube multiplex PCR and standard gel electrophoresis. Appl Environ Microbiol. 2019 Jan 04;: Authors: Caméléna F, Birgy A, Smail Y, Courroux C, Mariani-Kurkdjian P, Le Hello S, Bonacorsi S, Bidet P Abstract We developed a multiplex PCR method based on Multiple-Locus Variable-number of tandem repeats (VNTR)-Analysis (MLVA) designed for rapid typing of E. coli and Shigella isolates The method amplifying seven VNTRs doesn't require sequencing capillary nor fluorescent dyes. Amplification products are simply loaded on a standard agarose gel for electrophoresis and banding patterns are analyzed visually. We evaluated the method on 220 strains belonging to different collections: ECOR collection (n=72), O1:K1 isolates causing neonatal meningitis (n=38), extended-spectrum beta-lactamase-producing fecal isolates belonging to the worldwide ST131 clone (n=38), Shiga-toxin producing E. coli (STEC) of serogroups O157:H7 (n=21) and O26 (n=16, 8 belonging to an outbreak), 27 Shigella isolates (22 S. sonnei including 5 epidemic strains) and 8 reference strains. The performances were compared to MLST, the DiversiLab automated REP-PCR, PFGE and whole genome sequencing. On the ECOR collection, we found 66 different profiles. Among the clonal group O1:K1, 14 different profiles were identified. For the 37 STECs, we had 23 p...
Source: Applied and Environmental Microbiology - Category: Microbiology Authors: Tags: Appl Environ Microbiol Source Type: research

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Applied Physiology, Nutrition, and Metabolism, e-First Articles. We are currently facing an “obesity epidemic” worldwide. Promoting inefficient metabolism in muscle represents a potential treatment for obesity and its complications. Sarco(endo)plasmic reticulum (SR) Ca2+-ATPase (SERCA) pumps in muscle are responsible for maintaining low cytosolic Ca2+ concentration through the ATP-depe ndent pumping of Ca2+ from the cytosol into the SR lumen. SERCA activity has the potential to be a critical regulator of body mass and adiposity given that it is estimated to contribute upwards of 20% of daily energy expenditure....
Source: Applied Physiology, Nutrition, and Metabolism - Category: Physiology Authors: Source Type: research
Publication date: Available online 10 December 2019Source: Colloids and Surfaces B: BiointerfacesAuthor(s): Vahid Shirshahi, Seyed Nasrollah Tabatabaei, Shadie Hatamie, Reza SaberAbstractLateral flow immunoassay (LFA) is a well-known point-of-care technology for the detection of various analytes. However, low sensitivity and lack of quantitative results are some of its critical drawbacks. Here we report a photothermal enhanced lateral flow sensor on the basis of the photothermal properties of reduced graphene oxide (rGO) for the detection of E-coli O157:H7 as a model pathogen. The calibration curve of the photothermal meth...
Source: Colloids and Surfaces B: Biointerfaces - Category: Biochemistry Source Type: research
Publication date: February 2020Source: Phytochemistry Letters, Volume 35Author(s): Gamal A. Mohamed, Sabrin R.M. Ibrahim, Hani Z. AsfourAbstractTwo new diaryl ether derivatives [aspergillethers A (4) and B (5)] and five known metabolites [(22E,24R)-stigmasta-5,7,22-trien-3-β-ol (1), stigmasta-4,6,8(14),22-tetraen-3-one (2), orcinol (3), and butyrolactones I (6) and VI (7)] were separated from the endophytic fungus Aspergillus versicolor (Aspergillaceae) isolated from the roots of Pulicaria crispa Forssk (Asteraceae) growing in Saudi Arabia. The structures of these compounds were determined by various spectral techniqu...
Source: Phytochemistry Letters - Category: Chemistry Source Type: research
Publication date: 15 March 2020Source: Journal of Alloys and Compounds, Volume 817Author(s): Rui Guo, Aiguo Yan, Juanjuan Xu, Botao Xu, Tingting Li, Xuanwen Liu, Tingfeng Yi, Shaohua LuoAbstractBiVO4/CdS composites with different morphologies and mass ratios were successfully prepared via a facile deposition-precipitation method by adding different dispersants. Compared with spherical BiVO4 (BS), sheet-like BiVO4 nanoarrays (BA) showed higher (020) exposed areas, as confirmed by X-ray diffraction (XRD), scanning electron microscopy (SEM), and transmission electron microscopy (TEM). X-ray photoelectron spectroscopy (XPS) re...
Source: Journal of Alloys and Compounds - Category: Chemistry Source Type: research
Publication date: Available online 11 December 2019Source: EpidemicsAuthor(s): Theresa Stocks, Leah J. Martin, Sharon Kühlmann-Berenzon, Tom BrittonAbstractTo reach the WHO goal of hepatitis C elimination, it is essential to identify the number of people unaware of their hepatitis C virus (HCV) infection and to investigate the effect of interventions on the disease transmission dynamics. In many high-income countries, one of the primary routes of HCV transmission is via contaminated needles shared by people who inject drugs (PWIDs). However, substantial underreporting combined with high uncertainty regarding the size ...
Source: Epidemics - Category: Epidemiology Source Type: research
Publication date: Available online 11 December 2019Source: Medicina Intensiva (English Edition)Author(s): F.G. Márquez-Villegas, A. Elizalde-Figueroa, J.P. Venzor-Castellanos
Source: Medicina Intensiva - Category: Intensive Care Source Type: research
Publication date: January 2020Source: Journal of Photochemistry and Photobiology B: Biology, Volume 202Author(s): Rajasree Shanmuganathan, Felix LewisOscar, Sabarathinam Shanmugam, Nooruddin Thajuddin, Sulaiman Ali Alharbi, Naiyf S. Alharbi, Kathirvel Brindhadevi, Arivalagan PugazhendhiAbstractBacterial pathogenicity is becoming a major cause of morbidity and mortality around the globe. Researchers are tirelessly finding solutions to cure or prevent infections caused by bacterial pathogens. Nanotechnology is a fast-growing area of research, effectively influencing and preventing bacterial growth. Nanoparticles (NPs) of sil...
Source: Journal of Photochemistry and Photobiology B: Biology - Category: Speech-Language Pathology Source Type: research
Chairman of The Nippon Foundation Yohei Sasakawa and BRAC Executive Director Asif Saleh announcing $2 million partnership. Credit: Rafiqul Islam / IPSBy Rafiqul IslamDHAKA, Bangladesh, Dec 11 2019 (IPS) In the light of limited access to education for displaced Rohingya children, the Nippon Foundation has announced US$ 2 million support to BRAC to launch a project to ensure educational facilities to both Rohingya and local community children. The Nippon Foundation made the announcement at a press conference at the BRAC Centre in Dhaka, which was attended by Nippon Foundation chairman Yohei Sasakawa and BRAC Executive Direc...
Source: IPS Inter Press Service - Health - Category: International Medicine & Public Health Authors: Tags: Asia-Pacific Conferences Development & Aid Education Featured Headlines Health Human Rights Migration & Refugees Poverty & SDGs TerraViva United Nations National Leprosy Conference - Dhaka 2019 Source Type: news
There are at least eight cases of E. coli in three states linked to Fresh Express Sunflower Crisp Chopped Salad Kits. But it's unclear if these new cases are connected to a more widespread outbreak from romaine lettuce. Jonathan Vigliotti reports.
Source: Health News: CBSNews.com - Category: Consumer Health News Source Type: news
CONCLUSIONS: A complete recovery of cranial nerves VII and VIII in the course of RHS can be achieved. PMID: 31816219 [PubMed - as supplied by publisher]
Source: Neuroendocrinology Letters - Category: Endocrinology Tags: Neuro Endocrinol Lett Source Type: research
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