Inter-laboratory validation of the in-vivo flow cytometric micronucleus analysis method (MicroFlow®) in China

Publication date: 15 September 2014 Source:Mutation Research/Genetic Toxicology and Environmental Mutagenesis, Volume 772 Author(s): Yan Chang , Changhui Zhou , Fanghua Huang , Dorothea K. Torous , Yang Luan , Chang Shi , Hongling Wang , Xin Wang , Na Wei , Zhenna Xia , Zhaomin Zhong , Ming Zhang , Fengjun An , Yiyi Cao , Xingchao Geng , Ying Jiang , Qing Ju , Yongsheng Yu , Jiangbo Zhu , Stephen D. Dertinger , Bo Li , Mingyang Liao , Bojun Yuan , Tianbao Zhang , Jinbao Yu , Zhongchun Zhang , Qingli Wang , Jing Ma Although inter-laboratory validation efforts of the in-vivo micronucleus (MN) assay based on flow cytometry (FCM) have taken place in the EU and US, none have been organized in China. Therefore, an inter-laboratory study that included eight laboratories in China and one experienced reference laboratory in the US was coordinated to validate the in-vivo FCM MicroFlow® method to determine the frequency of micro-nucleated reticulocytes (MN-RETs) in rat blood. Assay reliability and reproducibility were evaluated with four known genotoxicants, and the results obtained with the FCM method were compared with the outcome of the traditional evaluation of bone-marrow micronuclei by use of microscopy. Each of the four chemicals was tested at three sites (two in China and the one US reference laboratory). After three consecutive daily exposures to a genotoxicant, blood and bone-marrow samples were obtained from rats 24h after the third dose. MN-R...
Source: Mutation Research Genetic Toxicology and Environmental Mutagenesis - Category: Genetics & Stem Cells Source Type: research