The Inverted U-Curve Association of Fluoride and Osteoclast Formation in Mice.

The Inverted U-Curve Association of Fluoride and Osteoclast Formation in Mice.
Biol Trace Elem Res. 2019 Jan 02;:
Authors: Yao Y, Ma Y, Zhong N, Pei J
Abstract
The effect of fluoride on osteoclasts is still controversial. In the past, researchers thought that the effects of fluoride on osteoclast and osteoblast formation occurred in a dose-dependent pattern. However, our previous in vitro study showed fluoride elicited a notably different effect on osteoclast formation. To further verify the relationship between fluoride and osteoclast formation in vivo, 60 male C57BL/6 mice were randomly divided into three groups: two treatment groups consuming water supplemented with 50 and 100 mg/L of fluoride, and a third control group with nonsupplemented water. Ion selective electrode method analysis was used to detect bone fluoride content, and the effects of fluoride on bone tissue were assessed with hematoxylin and eosin (HE) staining. Additionally, the expression of BGP and ALP were examined by Western blot analysis, and tartrate-resistant acid phosphatase (TRAP) was assessed with immunohistochemistry. Osteoclasts in bone tissue were identified with a combination method of TRAP staining and cell morphology assessment. Results showed increasing expression of BGP among treatment groups as fluoride exposure increased, and ALP expression in the 100 mg/L treatment group was significantly higher than that for both the 50 mg/L treatment and con...

Source: Biological Trace Element Research - January 2, 2019 Category: Biology Authors: Yao Y, Ma Y, Zhong N, Pei J Tags: Biol Trace Elem Res Source Type: research