Nanostars on Nanopipettes: A Raman Probe for Quantifying Oxygen Levels in Hypoxia in Single Cells and Tumors.

Nanostars on Nanopipettes: A Raman Probe for Quantifying Oxygen Levels in Hypoxia in Single Cells and Tumors. Angew Chem Int Ed Engl. 2019 Jan 02;: Authors: Lee SY Abstract Multiple sharp-edged gold nanostars were efficiently assembled on nanopipett tips by 3-aminopropyltrimethoxysilane (APTES) via an electrostatic interaction for a potent intracellular hypoxia-sensing Raman probe. Colloidal stability and surface immobiization was checked using scanning electron microscopy, light scattering and zeta potential measurements. Site-specific intracellular hypoxia levels can be estimated in vitro and in vivo using Raman lancets (RL). Distinct Raman spectral changes for the nitro-(NO2) functional group of the redox maker 4-nitrothiophenol (4NTP) can be quantified according to the intracellular oxygen (O2) contents, ranging from 1% to 10%. Facile removal of RL from cells can be achieved after a short measurement time. Redox potential changes in mitochondrial respiration could also be examined using serial injection of inhibitors. Three-dimensional (3D)-cultured cells and in vivo tests were used to validate our methods of measuring tumour hypoxia; potential applications were validated in terms of judging the aggressiveness of cancer cells by differentiating spectral changes between malignant and benign cells. PMID: 30600872 [PubMed - as supplied by publisher]
Source: Angewandte Chemie - Category: Chemistry Authors: Tags: Angew Chem Int Ed Engl Source Type: research