Generation of induced pluripotent stem cell line, ICGi007-A, by reprogramming peripheral blood mononuclear cells from a patient with Huntington's disease

Publication date: Available online 2 January 2019Source: Stem Cell ResearchAuthor(s): E.V. Grigor'eva, T.B. Malankhanova, A. Surumbayeva, J.M. Minina, V.V. Morozov, Abramycheva N. Yu, S.N. Illarioshkin, A.A. Malakhova, S.M. ZakianAbstractHuntington's disease (HD) is an autosomal dominant neurodegenerative disease caused by mutation in the HTT gene encoding HTT protein. The mutant protein leads to the neuronal death through dysregulation of multiple cellular processes. HD human induced pluripotent stem cells (iPSCs) represent a useful and valid model for the disease study. iPSC line from HD patient with 47 CAG repeats in HTT was generated from blood mononuclear cells by non-integrating episomal vectors. The iPSC line retained the mutation, expressed pluripotency markers, had a normal karyotype and displayed in vitro differentiation to the three germ layers.Resource table.Unique stem cell lines identifierICGi007-AAlternative name(s) of stem cell line47Q-3LfInstitutionFederal Research Center Institute of Cytology and Genetics, the Siberian Branch of the Russian Academy of Sciences, Novosibirsk, RussiaContact information of distributorElena V. Grigor'evaType of cell linesiPSCOriginHumanAdditional origin infoAge: 27Sex: FEthnicity: CaucasianCell SourcePeripheral blood mononuclear cellsClonalityClonalMethod of reprogrammingTransgene free episomal plasmid vectorsGenetic ModificationYESType of ModificationHereditaryAssociated diseaseHuntington's diseaseGene/locus4p16.3Method of modif...
Source: Stem Cell Research - Category: Stem Cells Source Type: research