The SNAP25 interactome in ventromedial caudate in schizophrenia includes the mitochondrial protein ARF1

Publication date: Available online 2 January 2019Source: NeuroscienceAuthor(s): Alfredo Ramos-Miguel, Vilte Barakauskas, Jehan Alamri, Masatoshi Miyauchi, Alasdair M. Barr, Clare L. Beasley, Gorazd Rosoklija, J. John Mann, Andrew J. Dwork, Annie Moradian, Gregg B. Morin, William G. HonerAbstractAbnormalities of SNAP25 (synaptosomal-associated protein 25) amount and protein-protein interactions occur in schizophrenia, and may contribute to abnormalities of neurotransmitter release in patients. However, presynaptic terminal function depends on multiple subcellular mechanisms, including energy provided by mitochondria. To explore the SNAP25 interactome in schizophrenia, we immunoprecipitated SNAP25 along with interacting proteins from the ventromedial caudate of 15 cases of schizophrenia and 13 controls. Proteins were identified with mass spectrometry-based proteomics. As well as 15 SNARE- (soluble N-ethylmaleimide-sensitive factor attachment protein receptor) associated proteins, we identified 17 mitochondrial-associated and 4 other proteins. The mitochondrial small GTPase ARF1 (ADP-ribosylation factor 1) was identified in 8 schizophrenia SNAP25 immunoprecipitates and none from controls (P = 0.004). Although the ARF1-SNAP25 interaction may be increased, immunoblotting demonstrated 21% lower ARF1-21 (21 kiloDaltons) in schizophrenia samples (P = 0.04). In contrast, the mitochondrial protein UQCRC1 (ubiquinol-cytochrome c reductase core protein 1) did not differ. Lower ARF1-21 le...

Source: Neuroscience - January 3, 2019 Category: Neuroscience Source Type: research