Limitations and Confusing Aspects of Diagnostic Testing for Neurologic Lyme Disease in the United States Minireviews

In the United States, laboratories frequently offer multiple different assays for testing of cerebrospinal fluid (CSF) samples to provide laboratory support for the diagnosis of central nervous system Lyme disease (CNSLD). Often included among these diagnostic tests are the same enzyme immunoassays and immunoblots that are routinely used to detect the presence of antibodies to Borrelia burgdorferi in serum. However, performing these assays on CSF alone may yield positive results simply from passive diffusion of serum antibodies into the CSF. In addition, such tests are only U.S. Food and Drug Administration cleared and well validated for testing serum, not CSF. When performed using CSF, positive results from these assays do not establish the presence of intrathecal antibody production to B. burgdorferi and therefore should not be offered. The preferred test to detect intrathecal production of antibodies to B. burgdorferi is the antibody index assay, which corrects for passive diffusion of serum antibodies into CSF and requires testing of paired serum and CSF collected at approximately the same time. However, this assay also has limitations and should only be used to establish a diagnosis of CNSLD in conjunction with patient exposure history, clinical presentation, and other laboratory findings.
Source: Journal of Clinical Microbiology - Category: Microbiology Authors: Tags: Minireviews Source Type: research

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This study aimed to investigate the presence and perform molecular characterization of Anaplasmataceae, Rickettsia spp., and Borrelia burgdorferi sensu lato (sl) in tick species collected in Corsica. Ticks from cattle (Bos taurus), sheep (Ovis aries), and rodents (Rattus rattus) were collected from May to September 2016. DNA was purified from ticks, submitted to quantitative real-time polymerase chain reaction (qPCR) and sequenced for phylogenetic analysis. In total, 660 ticks were collected from 111 animals during the study. The most abundant collected tick species from cattle was Rhipicephalus bursa (n = 49...
Source: Ticks and Tick borne Diseases - Category: Zoology Source Type: research
Authors: Boulanger N, Boyer P, Talagrand-Reboul E, Hansmann Y Abstract Ticks are a major group of arthropod vectors, characterized by the diversity of pathogens they transmit, by their impact on human and animal health, and by their socioeconomic implication especially in countries of the Southern Hemisphere. In Europe, Ixodes is the most important tick due to its wide distribution in the ecosystems and the variety of transmitted pathogens, in particular Borrelia (responsible for Lyme borreliosis), but also the tick-borne encephalitis virus. Their increased presence in the environment since the beginning of the 20t...
Source: Medecine et Maladies Infectieuses - Category: Infectious Diseases Tags: Med Mal Infect Source Type: research
(Natural News) A mass migration of birds may be amazing to behold, until they leave countless disease-ridden ticks in their wake. In the U.S., the disease-laden ticks spread in the Northeast and Midwest through dogs which serve as carriers for the Borrelia burgdorferi infection. In 2008, Canadian veterinarians worked on a project which looked into an...
Source: - Category: Consumer Health News Source Type: news
Publication date: Available online 6 February 2019Source: International Journal for Parasitology: Parasites and WildlifeAuthor(s): Natalia M. Pukhovskaya, Olga V. Morozova, Nelya P. Vysochina, Nadejda B. Belozerova, Leonid I. IvanovAbstractBorrelia burgdorferi sensu lato (s.l.) DNA was detected by PCR in Ixodes persulcatus Schulze, 1930, Haemaphysalis concinna Koch, 1844, Haemaphysalis japonica douglasi Nuttall et Warburton, 1915 and Dermacentor silvarum Olenev, 1932 ticks collected in the Amur region, the Jewish Autonomous region, the Sakhalin region and on the Khabarovsk territory. Infection rate of I. persulcatus with B...
Source: International Journal for Parasitology: Parasites and Wildlife - Category: Parasitology Source Type: research
Publication date: Available online 4 February 2019Source: Veterinary MicrobiologyAuthor(s): Alena Žákovská, Hana Lya Kučerová, Jiřina Marková, Eva BártováAbstractThe aim of of this study was to determine the seroprevalence of antibodies to B. burgdorferi s.l. in wild small mammals in the Czech Republic and compare sensitivity of PCR and cultivaton. Wild small mammals (n = 691) were trapped in years 2010 – 2014 in three localities of the Czech Republic. Heart rinses (n = 340) and sera (n = 351) were examined by modified indirect ELI...
Source: Veterinary Microbiology - Category: Veterinary Research Source Type: research
CONCLUSION: In the presence of unilateral or bilateral diaphragmatic paralysis of undetermined aetiology, it seems relevant to perform Lyme serology in the blood and, in positive cases, to follow up with a lumbar puncture in order to detect intrathecal IgG synthesis. PMID: 30711345 [PubMed - as supplied by publisher]
Source: Revue des Maladies Respiratoires - Category: Respiratory Medicine Tags: Rev Mal Respir Source Type: research
e;a D, Labruna MB Abstract Microorganisms harbored by Chilean autochthonous ticks have been scarcely studied and current knowledge is restricted to three species of hard ticks only. The current study aimed to assess the presence of Anaplasmataceae, Borrelia and Hepatozoon agents in ticks collected directly from the environment, on reptiles, birds and mammals in twelve localities from northern, central and southern regions of the country and Antarctica. Ticks were identified by means of a morphological and molecular approach. PCR detections point the occurrence of an Anaplasma-like agent and a relapsing fever Borre...
Source: Acta Tropica - Category: Infectious Diseases Authors: Tags: Acta Trop Source Type: research
Borrelia sp. is a causative pathogen of Lyme disease which has become a worldwide health concern. Non-toxic approaches especially directed toward latent persistent forms of this pathogen are desired. Lipids in th...
Source: BMC Complementary and Alternative Medicine - Category: Complementary Medicine Authors: Tags: Research article Source Type: research
Updated Date: Feb 1, 2019 EST
Source: DailyMed Drug Label Updates - Category: Drugs & Pharmacology Source Type: alerts
Purpose: Lyme borreliosis is a multisystem infection caused by the spirochete Borrelia burgdorferi. A disease can affect every organ, and it is characterized by a wide range of clinical manifestations. Erythema migrans is the basic clinical marker of Lyme disease in acute or subacute form. Recurrences occur in 9 –30% of inadequately treated patients. The aim of this paper was to investigate the frequency and clinical forms of skin borreliosis on our material, with the presentation of four atypical cases and to draw attention to the importance of clinical recognition of the disease.
Source: International Journal of Infectious Diseases - Category: Infectious Diseases Authors: Tags: 21.002 Source Type: research
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