Micro-solid phase extraction and LC–MS3 for the determination of triptorelin in rat plasma and application to a pharmacokinetic study

Publication date: 20 March 2019Source: Journal of Pharmaceutical and Biomedical Analysis, Volume 166Author(s): Tianming Ren, Zhi Zhang, John Paul Fawcett, Dong Sun, Jingkai GuAbstractTriptorelin is a synthetic decapeptide used for the treatment of prostate cancer. Attempts to determine triptorelin in clinical use by liquid chromatography-tandem mass spectrometry with multiple reaction monitoring have encountered problems due to its low concentration in plasma (pg/mL) and interference from endogenous peptides. We have overcome these issues using micro-solid phase extraction (μ-SPE) on Oasis® HLB 96-well μElution plates followed by LC–MS3. Sample preparation by μ-SPE achieved sample concentration without the need for evaporation and reconstitution steps. Detection by LC–MS3 showed no significant matrix interference at the retention time of analyte and achieved high sensitivity (lower limit of quantitation 10 pg/mL) and good linearity in the range 10–3000 pg/mL. The method was successfully applied to a pharmacokinetic study in rat involving a single intramuscular injection of a formulation of triptorelin acetate biodegradable microspheres.
Source: Journal of Pharmaceutical and Biomedical Analysis - Category: Drugs & Pharmacology Source Type: research