Cytogenotoxic effects of Adenium obesum seeds extracts on breast cancer cells

Publication date: Available online 30 December 2018Source: Saudi Journal of Biological SciencesAuthor(s): Ahmed Qasem Ali, Mohammad Abul Farah, Faisal M. Abou-Tarboush, Khalid M. Al-Anazi, M. Ajmal Ali, Joongku Lee, Waleed A. Q. Hailan, Ahmed Hossam MahmoudAbstractThe extracts prepared from various areal parts of the Adenium obesum (Forssk.) Roem. & Schult. (Family: Apocynaceae) including leaves, fruit and seeds ethanolic extracts and seed aqueous extract were evaluated against MCF-7 cells in order to investigate its potential of cytogenotoxicity and induction of apoptosis. The ethanolic seeds extract had comparatively higher cytotoxicity (IC50 ∼ 337 µg/ ml). Further, apoptosis and DNA damaging potential of seeds ethanolic extract was analyzed by applying multiple sub-lethal concentrations and durations. Flow cytometry results revealed that maximum percentage of early apoptosis (37%) and late apoptosis (35%) were observed after 12 h exposure in concentrations 200 µg/ ml and 300 µg/ ml, respectively. Similarly, the higher effect of extract in terms of DNA damage by alkaline comet assay was registered after 12 h treatment at concentrations 200 and 300 µg/ mL. The calculated total damage score (TDS) for these concentrations were 614 and 617, respectively. The above findings indicate that A. obesum ethanolic seeds extracts has cytogenotoxic properties that could be further explored for the potential source of chemotherapeutic lead against cancer.
Source: Saudi Journal of Biological Sciences - Category: Biology Source Type: research