Electrooptical determination of the isoelectric point of globular proteins: cytochrome c adsorbed on montmorillonite nanoplates

Publication date: Available online 30 December 2018Source: Colloids and Surfaces B: BiointerfacesAuthor(s): Svetlana H. Hristova, Alexandar M. ZhivkovAbstractThe purpose is to study the capability of the electric light scattering to determine the point of zero charge of native protein macromolecules adsorbed on colloid particles at low ionic strength and without using buffers. The chosen protein and particles are cytochrome c (cytC, globular haemoproteid) and montmorillonite (MM, negatively charged plate-like crystal). The pH-dependence of the electric polarizability γ(pH) in the range pH 6–11 shows minimum which coincides with the isoelectric point determined by measuring the electrophoretic mobility μ(pH) of the cytC-MM particles.Graphical abstract
Source: Colloids and Surfaces B: Biointerfaces - Category: Biochemistry Source Type: research