Purification, crystallization and preliminary X-ray crystallographic studies of KstR2 (ketosteroid regulatory protein) from Mycobacterium tuberculosis

KstR2 (Rv3557c) is one of two TetR-family transcriptional repressors of cholesterol metabolism in Mycobacterium tuberculosis. The ability to degrade cholesterol fully is important for pathogenesis, and therefore this repressor was expressed, purified and crystallized. Crystals of KstR2 diffracted to better than 1.9 Å resolution and belonged to space group C2, with unit-cell parameters a = 72.3, b = 90.3, c = 49.7 Å, α = γ = 90, β = 128.2°.

Source: Acta Crystallographica Section F - November 14, 2014 Category: Biochemistry Authors: Dawes, S.S.Kendall, S.L.Baker, E.N.Lott, J.S. Tags: cholesterol TetR family Mycobacterium tuberculosis steroid crystallization communications Source Type: research