Direct Stochastic Optical Reconstruction Microscopy (dSTORM)

Single-molecule localization-based super-resolution microscopy can be performed with regular, bright, and photostable organic fluorophores. We review a concept termed direct stochastic optical reconstruction microscopy (dSTORM), which operates conventional fluorophores as photoswitches and provides an optical resolution of ~20 nm. We introduce the principle of dSTORM, illustrate experimental schemes, and discuss approaches for data analysis.

Source: Springer protocols feed by Imaging/Radiology - November 13, 2014 Category: Radiology Source Type: news