Heparin Appended ADH-Anionic Polysaccharide Nanoparticles for Site-Specific Delivery of Usnic Acid

Publication date: Available online 27 December 2018Source: International Journal of PharmaceuticsAuthor(s): Ashish Garg, Sweta Garg, Nitendra K. Sahu, Sarita Rani, Umesh Gupta, Awesh K. YadavAbstractThe intention of present research work is to formulate usnic acid (UA) loaded heparin modified gellan gum (HAG) nanoparticles (NPs). HAG copolymer based conjugation was synthesized and characterized by 1H-NMR and FT-IR spectroscopy. Plain and UA loaded HAG NPs were prepared via nanoprecipitation technique. NPs were typified and further characterized for particle size, polydispersity index, entrapment efficiency, zeta potential, atomic force microscopy, differential scanning calorimetry, X-ray diffraction analysis, and in-vitro release. In-vitro tube formation assay, tumorsphere assay, autophagy assay, DNA cleavage assay, internalization by confocal and FACS based internalization analysis, caspase assay and cell cycle assay were performed for biological activity. Obtained experimental results explored that HAG NPs displayed a sustained release of UA (95.67% in 48 hrs) compared to gellan gum NPs (96.12% in 8 hrs). In cytotoxicity studies, UA loaded HAG NPs exhibited an enormous cytotoxic potential against A549 cancer cells. In the in vivo bio-distribution study, using albino rat model the free UA concentration was found 7.09±0.9%, 2.7±1.5%, 7.5±2.1, 9.2±2%, and 6.25±1.3% post two hours of intravenous administration, however, in the case of UA loaded HAG NPs ...
Source: International Journal of Pharmaceutics - Category: Drugs & Pharmacology Source Type: research