Antibacterial Silk Fibroin Scaffolds with Green Synthesized Silver Nanoparticles for Osteoblast Proliferation and Human Mesenchymal Stem Cell Differentiation

We report here, a silk fibroin based bone tissue engineered scaffold with AgNPs having advanced antimicrobial properties, without compromising its cytocompatibility and stem cell differentiation potential. For this purpose, AgNPs were in situ synthesized using silk fibroin as reducing as well as stabilizing agent. The antimicrobial activity of silk fibroin films with AgNPs was evaluated against gram negative bacteria as well as antibiotic resistant bacteria and it was found to be effective against both. The cytocompatibility of these scaffolds was examined with fibroblast and osteoblast cells. Also the effect of AgNPs present in films, on osteogenic differentiation potential of human mesenchymal stem cells was studied and it was observed that the presence of AgNPs at lower concentrations did not have any detrimental effect.Graphical Abstract
Source: Colloids and Surfaces B: Biointerfaces - Category: Biochemistry Source Type: research