Primary Human Brain Pericytes

Pure and PotentThe authors of a chapter on pericytes published in SpringerLink referred to ourhuman primary pericytes as " pericyte-like " , but chose not to characterize them because of the " exorbitant cost " . Dore-Duffy P., Esen N. (2018) The Microvascular Pericyte: Approaches to Isolation, Characterization, and Cultivation. In: Birbrair A. (eds) Pericyte Biology - Novel Concepts. Advances in Experimental Medicine and Biology, vol 1109. Springer, ChamGiven that these pericytes are also part of our hot selling3-D Human BBB Model, we are in the process of challenging these claims. We will always take inaccurate claims regarding our solutions seriously, and we take immediate action.Characterization of cells-we validated several key markers by immunofluorescence.Staining of Desmin (dilution 1:100). Secondary antibody conjugated to Alexa 594 (red) and counterstained with DAPI (blue). Cells were mounted using iBrite mounting media.Staining of Actin (dilution 25 ug/mL). Secondary antibody conjugated to Alexa 594 (red) and counterstained with DAPI (blue). Cells were mounted using iBrite mounting media.We also plan on doing a phenotypic analysis of these cells and will post here when completed.Cost of cells-isolating pericytes from human donors, and expanding to the required number of cells involves much time and effort. We know! Further differentiating stem cells into pericytes is hard and time-consuming. Against this backdrop, we consider ...
Source: Neuromics - Category: Neuroscience Tags: 3-D Blood-Brain Barrier Model Actin Antibody BBB Model Blood Brain Barrier Models Brain Perivascular Cells Desmin Antibody Human Brain Pericytes Immunofluorescence Source Type: news

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