Assessment of the calcium releasing machinery in oocytes that failed to fertilize after conventional ICSI and assisted oocyte activation

ConclusionsThe evaluation of spindle-chromosome normalcy and distribution of IP3R1 does not contribute to identify the presence of Ca2+ releasing deficiencies in these oocytes. However, oocyte Ca2+ analysis is of added value to identify Ca2+releasing incapacity of oocytes that failed to fertilize after ICSI or ICSI-AOA. We showed that some patients experiencing fertilization failure after ICSI-AOA present with a suspected activation deficiency downstream of the Ca2+ machinery, which cannot be overcome by ICSI-AOA based on the use of Ca2+ ionophores.
Source: Reproductive BioMedicine Online - Category: Reproduction Medicine Source Type: research