Reactive green dye remediation by Alternanthera philoxeroides in association with plant growth promoting Klebsiella sp. VITAJ23; A pot culture study

Publication date: Available online 21 December 2018Source: Microbiological ResearchAuthor(s): Astha Sinha, Sajitha Lulu S, Vino S, W.Jabez OsborneAbstractContamination of soil by textile effluent is a major threat found worldwide. These pollutants have a diverse negative effects on the ecosystem, therefore restoration through a cost biological strategy is the need of the hour. The aim of the current study was to enhance the decolourization of reactive green dye (RGD) by phytoremediation coupled with augmentation of effective bacteria to the rhizosphere. The isolate Klebsiella sp. VITAJ23 was isolated from textile polluted soil and was assessed for plant growth promoting traits (PGP) and the PGP functional genes were amplified. The soil was artificially polluted with RGD concentration ranging from 1000-3000 mg kg-1 and Alternanthera philoxeroides plantlets were planted in phyto and rhizoremediation treatments, setup was maintained upto 60 d. The isolate VITAJ23 was augmented in the rhizoremediation setup and the morphological were assessed at regular interval. There was a significant increase in the chlorophyll content as well as root and shoot length of the plant treated with bacterial suspension. Decolourization study revealed 79% removal of reactive green dye with an enhanced oxido-reductase enzyme activity in the plants bioaugmented with bacteria. The biodegraded metabolites were identified as 2-allylnapthalene, l-alanine, n-acetyl-and propenoic acid by GC-MS analysis ...
Source: Microbiological Research - Category: Infectious Diseases Source Type: research