Generation of a homozygous CRISPR/Cas9-mediated knockout human iPSC line for the STUB1 locus

Publication date: Available online 21 December 2018Source: Stem Cell ResearchAuthor(s): Stefanie Schuster, Srinethe Saravanakumar, Ludger Schöls, Stefan HauserAbstractSTUB1/CHIP is a central component of cellular protein homeostasis and interacts with key proteins involved in the pathogenesis of many neurodegenerative diseases. Missense and truncating mutations in STUB1 lead to SCAR16. For ideal in vitro disease modelling with isogenic controls, we generated a CHIP knockout cell line from a healthy control with no CHIP functionality, but remaining genomic integrity and verified pluripotency.
Source: Stem Cell Research - Category: Stem Cells Source Type: research