Survey of the analysis of continuous conformational variability of biological macromolecules by electron microscopy

Single-particle analysis by electron microscopy is a well established technique for analyzing the three-dimensional structures of biological macromolecules. Besides its ability to produce high-resolution structures, it also provides insights into the dynamic behavior of the structures by elucidating their conformational variability. Here, the different image-processing methods currently available to study continuous conformational changes are reviewed.

Source: Acta Crystallographica Section F - December 22, 2018 Category: Biochemistry Authors: Sorzano, C.O.S. Jim é nez, A. Mota, J. Vilas, J.L. Maluenda, D. Mart í nez, M. Ram í rez-Aportela, E. Majtner, T. Segura, J. S á nchez-Garc í a, R. Rancel, Y. del Ca ñ o, L. Conesa, P. Melero, R. Jonic, S. Vargas, J. Cazals, F. Freyberg, Z. Krieger, Tags: electron microscopy image processing continuous heterogeneity single-particle analysis normal-mode analysis research communications Source Type: research