MicroRNA-34a attenuates VEGF-mediated retinal angiogenesis via targeting the Notch1.

MicroRNA-34a attenuates VEGF-mediated retinal angiogenesis via targeting the Notch1. Biochem Cell Biol. 2018 Dec 20;: Authors: Shi S, Jin Y, Song H, Chen X Abstract Pathological angiogenesis in retina is main pathogenesis of ocular diseases closely associated with vision loss. Present work aimed to investigate effects of microRNA-34a (miR-34a) and its potential target Notch1 on retinal angiogenesis. For this objective, we employed oxygen-induced retinopathy (OIR) rats and human retinal microvascular endothelial cells (HRMECs) stimulated with vascular endothelial growth factors (VEGF). H&E staining, western blot for VEGF and immunofluorescence staining for CD31 were performed to verify establishment of OIR model. We observed down-regulation of miR-34a and up-regulation of Notch1 and Hey1 in retina from OIR rats. Similar results were got in VEGF-stimulated HRMECs. By performing MTT assay, cell scratch assay, tube formation assay and detecting expression of matrix-metalloproteinase-2 (MMP-2), MMP-9, tissue inhibitors of metalloproteinases-1 (TIMP-1) and TIMP-2, we found that transfection of miR-34a ameliorated VEGF-mediated angiogenesis of HRMECs. We further observed that siRNA-induced gene silencing of Notch1 prevented VEGF-induced angiogenesis via regulating cell proliferation, cell migration and tube formation of HRMECs Additionally, activation of Notch1 by transfection of Notch1 plasmid attenuated anti-tube formation effects of ...
Source: Biochemistry and Cell Biology - Category: Biochemistry Authors: Tags: Biochem Cell Biol Source Type: research