Dose-dependent differential resistance of inbred chicken lines to avian pathogenic Escherichia coli challenge.

Dose-dependent differential resistance of inbred chicken lines to avian pathogenic Escherichia coli challenge. Avian Pathol. 2018 Dec 20;:1-33 Authors: Alber A, Costa T, Chintoan-Uta C, Bryson KJ, Kaiser P, Stevens MP, Vervelde L Abstract Avian pathogenic E. coli (APEC) cause severe respiratory and systemic disease. To address the genetic and immunological basis of resistance, inbred chicken lines were used to establish a model of differential resistance to APEC, using strain O1 of serotype O1:K1:H7. Inbred lines 72, 15I and C.B12 and the outbred line Novogen Brown were inoculated via the airsac with a high dose (107 colony-forming units, CFU) or low dose (105 CFU) of APEC O1. Clinical signs, colibacillosis lesion score and bacterial colonisation of tissues after high dose challenge were significantly higher in line 15I and C.B12 birds. The majority of the 15I and C.B12 birds succumbed to the infection by 14 hours post-infection, whilst none of the line 72 and the Novogen Brown birds developed clinical signs. No difference was observed after low dose challenge. In a repeat study, inbred lines 72 and 15I were inoculated with low, intermediate or high doses of APEC O1 ranging from 105 to 107 CFU. The colonisation of lung was highest in line 15I after high dose challenge and birds developed clinical signs, however colonisation of blood and spleen, clinical signs and lesion score were not different between lines. No difference was observ...
Source: Avian Pathology - Category: Pathology Authors: Tags: Avian Pathol Source Type: research