Metabolic engineering of Corynebacterium glutamicum for the fermentative production of halogenated tryptophan

In this study, fermentative production of the halogenated amino acid 7-chloro-L-tryptophan from sugars, ammonium and chloride salts was achieved. This required metabolic engineering of an L-tryptophan producing C. glutamicum strain for expression of the genes coding for FAD-dependent halogenase RebH and NADH-dependent flavin reductase RebF from Lechevalieria aerocolonigenes. Chlorination of L-tryptophan to 7-chloro-L-tryptophan by recombinant C. glutamicum was improved by optimizing the RBS of rebH. Metabolic engineering enabled production of 7-chloro-L-tryptophan and L-tryptophan from the alternative carbon sources arabinose, glucosamine and xylose.
Source: Journal of Biotechnology - Category: Biotechnology Source Type: research