Synergetic Effect of Vancomycin Loaded Silver Nanoparticles for Enhanced Antibacterial Activity

Publication date: Available online 18 December 2018Source: Colloids and Surfaces B: BiointerfacesAuthor(s): Amritpal Kaur, Simran Preet, Vivek Kumar, Rajeev Kumar, Rajesh KumarAbstractIn recent years, there is significant growth in the bacterial resistance to various classic antibiotics. This has opened and enhanced the field of metal nanoparticles and antibiotic-metal nanoparticle complex. This research was designed to load a glycopeptide antibiotic named vancomycin on citrate-capped silver nanoparticles to enhance its antibacterial activity against both Gram-positive (Staphylococcus aureus) and Gram-negative (Escherichia coli) bacteria. Colloidal solution of silver nanoparticles (AgNPs) was prepared by chemical reduction method using silver nitrate (AgNO3) as a precursor in the presence of ionic surfactant trisodium-citrate which acts as a both capping and reducing agent. Synthesized nanoparticles were functionalized with vancomycin to form nano-drug complex (Van@AgNPs). Various analytical techniques such as UV-vis absorption spectra, FTIR, DLS, TEM and XRD were carried out to study the loading and interaction of drug with silver nanoparticles. The observed shift in SPR peak of UV-vis and various reflections of XRD spectra is attributed towards the loading of vancomycin on silver nanoparticle surface. FTIR studies shows the hydrogen bonding between vancomycin and silver nanoparticles through -NH- (amine) group of vancomycin and oxygen of anionic citrate. The increase in ave...
Source: Colloids and Surfaces B: Biointerfaces - Category: Biochemistry Source Type: research